LOCUS       Exported                4396 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Suicide vector pMO90, complete sequence.
ACCESSION   FJ267588
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Jones-Carson J, Laughlin J, Hamad MA, Stewart AL, Voskuil MI, 
            Vazquez-Torres A.
  TITLE     Inactivation of [Fe-S] metalloproteins mediates nitric 
            oxide-dependent killing of Burkholderia mallei
  JOURNAL   PLoS ONE 3 (4), E1976 (2008)
  PUBMED    18398486
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Jones-Carson J, Laughlin J, Hamad MA, Stewart A, Voskuil M, 
            Vazquez-Torres A.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2008) Microbiology, UCD, 12800 E. 19th Ave., 
            Denver, CO 80045, USA
REFERENCE   3  (bases 1 to 4396)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Suicide vector pMO90"
                     /mol_type="other DNA"
                     /label=Burkholderia suicide vector
                     /note="Burkholderia suicide vector"
                     /db_xref="taxon:565106"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             201..995
                     /codon_start=1
                     /product="AphA"
                     /label=AphA
                     /note="kanamycin resistance marker"
                     /protein_id="ACI42372.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             1071..1787
                     /codon_start=1
                     /product="GFP"
                     /label=GFP
                     /note="reporter"
                     /protein_id="ACI42371.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     oriT            2111..2220
                     /note="incP origin of transfer"
     rep_origin      2519..3107
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3275..3303
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     CDS             3335..4258
                     /codon_start=1
                     /product="XylE"
                     /label=XylE
                     /note="reporter"
                     /protein_id="ACI42370.1"
                     /translation="MNKGVMRPGHVQLRVLDMSKALEHYVELLGLIEMDRDDQGRVYLK
                     AWTEVDKFSLVLREADEPGMDFMGFKVVDEDALRQLERDLMAYGCAVEQLPAGELNSCG
                     RRVRFQAPSGHHFELYADKEYTGKWGLNDVNPEAWPRDLKGMAAVRFDHALMYGDELPA
                     TYDLFTKVLGFYLAEQVLDENGTRVAQFLSLSTKAHDVAFIHHPEKGRLHHVSFHLETW
                     EDLLRAADLISMTDTSIDIGPTRHGLTHGKTIYFFDPSGNRNEVFCGGDYNYPDHKPVT
                     WTTDQLGKAIFYHDRILNERFMTVLT"