LOCUS       Exported                7108 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Burkholderia replicative vector pMo168, complete sequence.
ACCESSION   EU862244
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7108)
  AUTHORS   Hamad MA, Zajdowicz SL, Holmes RK, Voskuil MI.
  TITLE     An allelic exchange system for compliant genetic manipulation of the
            select agents Burkholderia pseudomallei and Burkholderia mallei
  JOURNAL   Gene 430 (1-2), 123-131 (2009)
  PUBMED    19010402
REFERENCE   2  (bases 1 to 7108)
  AUTHORS   Hamad MA, Zajdowicz SL, Holmes RK, Voskuil MI.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2008) Microbiology, University of Colorado Denver 
            School of Medicine, 19th Ave, Aurora, CO 80010, USA
REFERENCE   3  (bases 1 to 7108)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7108
                     /organism="Burkholderia replicative vector pMo168"
                     /mol_type="other DNA"
                     /db_xref="taxon:549785"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             201..995
                     /codon_start=1
                     /gene="aphA"
                     /product="Km R"
                     /label=aphA
                     /note="kanamycin resistance protein; selection marker"
                     /protein_id="ACF93717.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            201..995
                     /gene="aphA"
                     /label=aphA
     promoter        complement(2584..2686)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     misc_feature    2836..3855
                     /label=mob region
                     /note="mob region"
     rep_origin      4079..4848
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     rep_origin      4651..4662
     CDS             4849..5511
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /note="replication protein"
                     /protein_id="ACF93715.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     gene            4849..5511
                     /gene="repA"
                     /label=repA
     promoter        5982..6010
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     CDS             6042..6965
                     /codon_start=1
                     /gene="xylE"
                     /product="XylE"
                     /label=xylE
                     /note="reporter marker"
                     /protein_id="ACF93716.1"
                     /translation="MNKGVMRPGHVQLRVLDMSKALEHYVELLGLIEMDRDDQGRVYLK
                     AWTEVDKFSLVLREADEPGMDFMGFKVVDEDALRQLERDLMAYGCAVEQLPAGELNSCG
                     RRVRFQAPSGHHFELYADKEYTGKWGLNDVNPEAWPRDLKGMAAVRFDHALMYGDELPA
                     TYDLFTKVLGFYLAEQVLDENGTRVAQFLSLSTKAHDVAFIHHPEKGRLHHVSFHLETW
                     EDLLRAADLISMTDTSIDIGPTRHGLTHGKTIYFFDPSGNRNEVFCGGDYNYPDHKPVT
                     WTTDQLGKAIFYHDRILNERFMTVLT"
     gene            6042..6965
                     /gene="xylE"
                     /label=xylE