LOCUS       Exported                6127 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Burkholderia suicide vector pMo130, complete sequence.
ACCESSION   EU862243
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Hamad MA, Zajdowicz SL, Holmes RK, Voskuil MI.
  TITLE     An allelic exchange system for compliant genetic manipulation of the
            select agents Burkholderia pseudomallei and Burkholderia mallei
  JOURNAL   Gene 430 (1-2), 123-131 (2009)
  PUBMED    19010402
REFERENCE   2  (bases 1 to 6127)
  AUTHORS   Hamad MA, Zajdowicz SL, Holmes RK, Voskuil MI.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2008) Microbiology, University of Colorado Denver 
            School of Medicine, 19th Ave, Aurora, CO 80010, USA
REFERENCE   3  (bases 1 to 6127)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="Burkholderia suicide vector pMo130"
                     /mol_type="other DNA"
                     /db_xref="taxon:549784"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             201..995
                     /codon_start=1
                     /gene="aphA"
                     /product="Km R"
                     /label=aphA
                     /note="kanamycin resistance protein; selection marker"
                     /protein_id="ACF93713.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            201..995
                     /gene="aphA"
                     /label=aphA
     CDS             1071..2531
                     /codon_start=1
                     /gene="sacB"
                     /product="modified SacB"
                     /label=sacB
                     /note="counter selection marker"
                     /protein_id="ACF93714.1"
                     /translation="MTYSKQAVSRRHKRLALSAAALTAAACMSAHAQSDGATQAFAKET
                     NQKPYKETYGISHITRHDMLQIPEQQKNEKYQVSEFDSSTIKNISSAKGLDVWDSWPLQ
                     NADGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFD
                     ANDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNIN
                     GVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTE
                     DGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKV
                     MKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTG
                     PYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAP
                     SFLLNIKGKKTSVVKDSILEQGQLTVNK"
     gene            1071..2531
                     /gene="sacB"
                     /label=sacB
     terminator      2542..2587
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      3521..4064
                     /label=oriT
                     /note="oriT"
     oriT            3838..3947
                     /note="incP origin of transfer"
     rep_origin      4246..4834
                     /label=pUC19 oriV
                     /note="pUC19 oriV"
     promoter        5001..5029
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     CDS             5061..5984
                     /codon_start=1
                     /gene="xylE"
                     /product="XylE"
                     /label=xylE
                     /note="reporter marker"
                     /protein_id="ACF93712.1"
                     /translation="MNKGVMRPGHVQLRVLDMSKALEHYVELLGLIEMDRDDQGRVYLK
                     AWTEVDKFSLVLREADEPGMDFMGFKVVDEDALRQLERDLMAYGCAVEQLPAGELNSCG
                     RRVRFQAPSGHHFELYADKEYTGKWGLNDVNPEAWPRDLKGMAAVRFDHALMYGDELPA
                     TYDLFTKVLGFYLAEQVLDENGTRVAQFLSLSTKAHDVAFIHHPEKGRLHHVSFHLETW
                     EDLLRAADLISMTDTSIDIGPTRHGLTHGKTIYFFDPSGNRNEVFCGGDYNYPDHKPVT
                     WTTDQLGKAIFYHDRILNERFMTVLT"
     gene            5061..5984
                     /gene="xylE"
                     /label=xylE