LOCUS       Exported                3439 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMN10, complete sequence.
ACCESSION   GU217589
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Jiwaji M, Daly R, Gibriel A, Barkess G, McLean P, Yang J, Pansare K,
            Cumming S, McLauchlan A, Kamola PJ, Bhutta MS, West AG, West KL, 
            Kolch W, Girolami MA, Pitt AR.
  TITLE     Unique reporter-based sensor platforms to monitor signalling in 
            cells
  JOURNAL   PLoS ONE 7 (11), E50521 (2012)
  PUBMED    23209767
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Jiwaji M.
  TITLE     The Molecular Nose
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3439)
  AUTHORS   Jiwaji M.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2009) FBLS, Glasgow University, University Avenue,
            Glasgow G12 8QQ, UK
REFERENCE   4  (bases 1 to 3439)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Cloning vector pMN10"
                     /mol_type="other DNA"
                     /label=derived from Escherichia coli plasmid pGL3-Basic
                     /note="derived from Escherichia coli plasmid pGL3-Basic"
                     /db_xref="taxon:698777"
     5UTR           137..176
                     /label=HSV TK 5-UTR
                     /note="5 untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     regulatory      193..3394
                     /regulatory_class="promoter"
                     /note="derived from human thymidine kinase"
     misc_feature    194..316
                     /label=unique reporter sequence
                     /note="unique reporter sequence"
     regulatory      341..532
                     /regulatory_class="polyA_signal_sequence"
     polyA_signal    350..471
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(890..1478)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1649..2509)
                     /codon_start=1
                     /gene="Amp"
                     /product="Amp"
                     /label=Amp
                     /note="resistance to antibiotic ampicillin"
                     /protein_id="ADA13290.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(1649..2509)
                     /gene="Amp"
                     /label=Amp
     promoter        complement(2510..2614)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2641..3096
     regulatory      3227..3380
                     /regulatory_class="polyA_signal_sequence"
     polyA_signal    3227..3275
                     /note="synthetic polyadenylation signal"
     misc_feature    3289..3380
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     protein_bind    complement(3406..3439)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."