LOCUS       Exported                4266 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Saccharomyces cerevisiae vector pMM25, complete sequence.
ACCESSION   GQ988388
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Moscariello MM, Sutherland BM.
  TITLE     Transferring linear DNA fragments in the yeast Saccharomyces 
            cerevisiae: A new in vivo eukaryotic system that reveals deleterious
            combinations of DNA cluster damage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Moscariello MM, Sutherland BM.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2009) Biology, Brookhaven National Laboratory, 50 
            Bell Avenue, Upton, NY 11973, USA
REFERENCE   3  (bases 1 to 4266)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Saccharomyces cerevisiae vector pMM25"
                     /mol_type="other DNA"
                     /note="hybrid cassette His3-Ade2 cloned in pBluescript II 
                     SK"
                     /db_xref="taxon:683353"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            713..1659
                     /gene="ADE2"
                     /label=ADE2
     regulatory      713..885
                     /regulatory_class="promoter"
                     /gene="ADE2"
     CDS             886..1659
                     /codon_start=1
                     /gene="ADE2"
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /label=ADE2
                     /note="catalyzes a step in de novo purine nucleotide 
                     biosynthetic pathway"
                     /protein_id="ACX71835.1"
                     /translation="MDSRTVGILGGGQLGRMIVEAANRLNIKTVILDAENSPAKQISNS
                     NDHVNGSFSNPLDIEKLAEKCDVLTIEIEHVDVPTLKNLQVKHPKLKIYPSPETIRLIQ
                     DKYIQKEHLIKNGIAVTQSVPVEQASETSLLNVGRDLGFPFVLKSRTLAYDGRGNFVVK
                     NKEMIPEALEVLKDRPLYAEKWAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNICDLC
                     YAPARVPDSVQLKAKLLAENAIKSFPGCGIFGVEMF"
     gene            1660..2018
                     /gene="HIS3"
                     /label=HIS3
     regulatory      1660..1999
                     /regulatory_class="promoter"
                     /gene="HIS3"
     promoter        1812..1999
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             2000..2018
                     /codon_start=1
                     /gene="HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /label=HIS3
                     /note="catalyzes the sixth step in histidine biosynthesis"
                     /protein_id="ACX71836.1"
                     /translation="MRQSRK"
     primer_bind     complement(2025..2041)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2078..2096)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2117..2133)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2141..2157
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2165..2195)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2210..2231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2519..3107)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3278..4138)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4139..4243)
                     /gene="bla"
                     /label=AmpR promoter