LOCUS       Exported                6124 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector pMLS7, complete sequence.
ACCESSION   AY112734
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Lefebre MD, Valvano MA.
  TITLE     Construction and evaluation of plasmid vectors optimized for 
            constitutive and regulated gene expression in Burkholderia cepacia 
            complex isolates
  JOURNAL   Appl. Environ. Microbiol. 68 (12), 5956-5964 (2002)
  PUBMED    12450816
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Lefebre MD, Valvano MA.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2002) Microbiology and Immunology, University of 
            Western Ontario, Dental Sciences Building Rm 3000, London, ONT N6A 
            5C1, Canada
REFERENCE   3  (bases 1 to 6124)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Broad host range vector pMLS7"
                     /host="Burkholderia cepacia"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:198729"
     promoter        complement(836..938)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             complement(1088..2107)
                     /codon_start=1
                     /gene="mob"
                     /product="Mob"
                     /function="required for plasmid mobilization"
                     /label=mob
                     /protein_id="AAM63386.1"
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEHW
                     AASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKWLA
                     DKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAAAVA
                     DLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLGISKR
                     VETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALGP
                     LNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSKKPLLS"
     gene            complement(1088..2107)
                     /gene="mob"
                     /label=mob
     rep_origin      2331..3100
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     rep_origin      2903..2914
     CDS             3101..3763
                     /codon_start=1
                     /gene="rep"
                     /product="Rep"
                     /function="required for plasmid replication"
                     /label=rep
                     /protein_id="AAM63387.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     gene            3101..3763
                     /gene="rep"
                     /label=rep
     CDS             complement(4215..4451)
                     /codon_start=1
                     /gene="dhfrII"
                     /product="dihydrofolate reductase"
                     /label=dhfrII
                     /note="trimethoprim resistance determinant"
                     /protein_id="AAM63385.1"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     gene            complement(4215..4451)
                     /gene="dhfrII"
                     /label=dhfrII
     promoter        complement(4778..4806)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     regulatory      4792..5641
                     /regulatory_class="promoter"
                     /note="S7 ribosomal protein promoter region from 
                     Burkholderia sp. LB400"
     misc_feature    5642..5697
                     /note="multiple cloning site; EcoRI to HindIII"
     regulatory      5698..6123
                     /regulatory_class="terminator"
                     /note="rrnB"
     terminator      5900..5986
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6078..6105
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"