LOCUS       Exported                8381 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Fluorescent reporter vector pmKATE2rrnB, complete sequence.
ACCESSION   KF245454
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8381)
  AUTHORS   van Gestel J, Weissing FJ, Kuipers OP, Kovacs AT.
  TITLE     Density of founder cells affects spatial pattern formation and 
            cooperation in Bacillus subtilis biofilms
  JOURNAL   ISME J 8 (10), 2069-2079 (2014)
  PUBMED    24694715
REFERENCE   2  (bases 1 to 8381)
  AUTHORS   Kovacs AT.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2013) Terrestrial Biofilms Group, Friedrich 
            Schiller University of Jena, Neugasse 23, Jena, Thuringia 07743, 
            Germany
REFERENCE   3  (bases 1 to 8381)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8381
                     /organism="Fluorescent reporter vector pmKATE2rrnB"
                     /mol_type="other DNA"
                     /db_xref="taxon:1497425"
     promoter        106..210
                     /gene="bla"
                     /label=AmpR promoter
     CDS             211..1071
                     /codon_start=1
                     /gene="bla"
                     /product="Bla"
                     /label=bla
                     /note="ampicillin resistance protein"
                     /protein_id="AHZ33562.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            211..1071
                     /gene="bla"
                     /label=bla
     rep_origin      1242..1830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2077..2859)
                     /codon_start=1
                     /gene="spec"
                     /product="Spec"
                     /label=spec
                     /note="spectinomycin resistance"
                     /protein_id="AHZ33563.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRIN
                     SDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYPPK
                     REFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLTDIR
                     RAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEHVTLL
                     DIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(2077..2859)
                     /gene="spec"
                     /label=spec
     gene            complement(3968..5005)
                     /gene="amyE"
                     /label=amyE
                     /note="3` end"
     CDS             complement(5367..6017)
                     /codon_start=1
                     /gene="Cm"
                     /product="Cm"
                     /label=Cm
                     /note="chloramphenicol acetyl transferase"
                     /protein_id="AHZ33565.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     gene            complement(5367..6017)
                     /gene="Cm"
                     /label=Cm
     regulatory      6330..6728
                     /regulatory_class="promoter"
                     /gene="rrnB"
     gene            6330..6728
                     /gene="rrnB"
                     /label=rrnB
     CDS             6735..7427
                     /codon_start=1
                     /gene="mKATE2"
                     /product="mKATE2"
                     /label=mKATE2
                     /note="red fluorescent protein; derived from Entacmaea 
                     quadricolor"
                     /protein_id="AHZ33564.1"
                     /translation="SELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKAV
                     EGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMALKL
                     VGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYCD
                     LPSKLGHR"
     gene            6735..7427
                     /gene="mKATE2"
                     /label=mKATE2
     regulatory      7450..7538
                     /regulatory_class="terminator"
     regulatory      7545..7631
                     /regulatory_class="terminator"
     gene            complement(7747..8267)
                     /gene="amyE"
                     /label=amyE
                     /note="5` end"