LOCUS       Exported                4318 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMK-RQ_MXAN_1527_1531exp, complete sequence.
ACCESSION   KJ633122
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Lorenzen W.
  TITLE     A multifunctional enzyme is involved in bacterial ether lipid 
            biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Lorenzen W.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAR-2014) Molekulare Biotechnologie / Institut fur 
            Molekulare Biowissenschaften, Goethe Universtaet Frankfurt, 
            Max-von-Laue Strasse 9, Frankfurt am Main, Hessen 60438, Germany
REFERENCE   3  (bases 1 to 4318)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ??? v. ???
            Sequencing Technology :: unknown
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Cloning vector pMK-RQ_MXAN_1527_1531exp"
                     /mol_type="other DNA"
                     /db_xref="taxon:1494598"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             373..1401
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="MXAN_1527"
                     /protein_id="AHY21922.1"
                     /translation="MKLLVTGGTGFLGTHLVPRLVAAGHEVRLIGRSRPSGAPYAGTEY
                     VPGDLKNRDAVRRALEGVDAVYHLAGLVSFQPKDARKMFELHVDSTRELLRDVREAGVK
                     RVVLASTSGTIAVSKEAHVRDESADYPITVVGQWPYYLSKIYEEKLALAYCRKHEIPLV
                     VLNPSLLMGPGDDRLSSTWTVVKFLNREIPAMPGGGISFVDARDAADAFVQALTRGEVY
                     GRHLMGVNLSMKDFFQRLERLSGVPAPKLKLPSKVNVLGGKLLERWAKVRGTTPTLDPQ
                     EIDIGEHWFWLDASKAEAELGFRARDIQETLSETVQHIYGKMPPQSLPGTKGRLAELRE
                     NT"
     CDS             1417..2397
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="MXAN_1531"
                     /protein_id="AHY21923.1"
                     /translation="MRALITGAGGFLGMWLAKALAARGDSVTCLLRPGGDASGLAGLTY
                     TRVEGDVTVPSTLSAAVAGQDVVFHLAGARRGATRDDFMRVNAEGTRHLCEAMVAAGHR
                     PRLVLTGSLAACGPSTPTRPHVEEDAFHPHEWYGESKAEAERIAFSYGDRLPVTVSRPP
                     RILGPGDRENLPFFKMAQRGIRLELSGGPRPLTMVDVEDVVDILLLQATHPAAIGEAFF
                     CAGPGEPLSLEKVQDLGAQALGLTPRTVRVSPALLRAMATAADGVSQLTGRKLALSRKM
                     AKQLLAPAWTCSGAKAERLLGFHPQRDLADSIRRSVQWYREQGWL"
     rep_origin      2578..3245
                     /label=Col/E1 origin
                     /note="Col/E1 origin"
     rep_origin      complement(2634..3222)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3396..4190)
                     /codon_start=1
                     /product="KanR"
                     /label=KanR
                     /protein_id="AHY21924.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(4191..4295)
                     /gene="bla"
                     /label=AmpR promoter