LOCUS       Exported                7020 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Neisserial shuttle/reporter vector pMIDG100, complete sequence.
ACCESSION   AY174111
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7020)
  AUTHORS   Webb SA, Langford PR, Kroll JS.
  TITLE     A promoter probe plasmid based on green fluorescent protein : a 
            strategy for studying meningococcal gene expression
  JOURNAL   Methods Mol. Med. 67, 663-677 (2001)
  PUBMED    21337172
REFERENCE   2  (bases 1 to 7020)
  AUTHORS   Webb SA, O Dwyer CA, Mauchline M, Langford PR, Kroll JS.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2002) Paediatrics, Imperial College, Norfolk 
            Place, London W2 1PG, UK
REFERENCE   3  (bases 1 to 7020)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7020
                     /organism="Neisserial shuttle/reporter vector pMIDG100"
                     /mol_type="genomic DNA"
                     /note="pJSK411; broad-host-range shuttle reporter plasmid 
                     derived from plasmid pLES2; described by Stein et al. 
                     (1993) Gene 25: 241-247"
                     /db_xref="taxon:217538"
     regulatory      12..39
                     /regulatory_class="terminator"
     misc_feature    47..106
                     /note="synthetic polylinker cloning domain; contains 
                     restriction sites XhoI, HindIII, EcoRI, SalI, ApaI, BamHI, 
                     and NheI"
     misc_feature    47..99
                     /label=MCS
                     /note="multiple cloning site"
     CDS             133..849
                     /codon_start=1
                     /gene="GFPmut3"
                     /product="green fluorescent protein mut3 variant"
                     /label=GFPmut3
                     /protein_id="AAO20110.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     gene            133..849
                     /gene="GFPmut3"
                     /label=GFPmut3
     regulatory      857..883
                     /regulatory_class="terminator"
     primer_bind     complement(920..936)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    944..960
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(968..998)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1013..1034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    1136..5642
                     /note="domain similar to gonococcal plasmid pJD4 sequence 
                     deposited in GenBank Accession Number U20374"
     CDS             complement(5715..6509)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=aphA-3
                     /note="AphA-3"
                     /protein_id="AAO20111.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCIRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(5715..6509)
                     /gene="aphA-3"
                     /label=aphA-3