LOCUS       Exported                4469 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMG401, complete sequence.
ACCESSION   KR360752
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Emge P.
  TITLE     Quorum sensing resilience against fluid flow
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   Emge P.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2015) Health Science and Technology, ETH Zurich, 
            Vladimir-Prelog-Weg 1-5/10, Zurich 8093, Switzerland
REFERENCE   3  (bases 1 to 4469)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Cloning vector pMG401"
                     /mol_type="other DNA"
                     /label=sequence provided by Prof. Francis Arnold, Caltech
                     /note="sequence provided by Prof. Francis Arnold, Caltech"
                     /db_xref="taxon:1735269"
     RBS             793..804
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     RBS             985..996
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     RBS             1713..1724
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1731..2486
                     /codon_start=1
                     /product="GFP-LVA"
                     /label=GFP-LVA
                     /note="Aequoria victoria green fluorescent protein with LVA
                     tag"
                     /protein_id="ALI16837.1"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYKRPAANDENYALVA"
     CDS             1731..2444
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      2535..2621
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(2791..3336)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      3450..3544
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3575..4369)
                     /codon_start=1
                     /gene="aph(3)-II"
                     /product="aminoglycoside phosphotransferase"
                     /label=aph(3)-II
                     /note="NeoR/KanR; derived from Tn5; confers resistance to 
                     neomycin, kanamycin, and G418 (Geneticin(R))"
                     /protein_id="ALI16836.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            complement(3575..4369)
                     /gene="aph(3)-II"
                     /label=aph(3)-II
                     /note="nptII"