LOCUS       Exported                5680 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMG103 DNA, complete sequence.
ACCESSION   AB031077
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Inui M, Roh JH, Zahn K, Yukawa H.
  TITLE     Sequence analysis of the cryptic plasmid pMG101 from 
            Rhodopseudomonas palustris and construction of stable cloning 
            vectors
  JOURNAL   Appl. Environ. Microbiol. 66 (1), 54-63 (2000)
  PUBMED    10618203
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Inui M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-1999) Masayuki Inui, Research Institute of 
            Innovative Technology for the Earth, Molecular Microbiology and 
            Genetics Laboratory; 9-2, Kizugawadai, Soraku, Kizu, Kyoto 619-0292,
            Japan (E-mail:inui@rite.or.jp, Tel:81-774-75-2308, 
            Fax:81-774-75-2321)
REFERENCE   3  (bases 1 to 5680)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Cloning vector pMG103"
                     /focus
                     /mol_type="other DNA"
                     /db_xref="taxon:102152"
     source          1..27
                     /organism="synthetic construct"
                     /mol_type="other DNA"
                     /label=derived from cloning vector pHSG298
                     /note="derived from cloning vector pHSG298"
                     /db_xref="taxon:32630"
     source          28..3080
                     /organism="Rhodopseudomonas palustris"
                     /plasmid="pMG101"
                     /mol_type="other DNA"
                     /label=derived from 3.0-kb XhoI-SalI fragment of pMG101
                     /note="derived from 3.0-kb XhoI-SalI fragment of pMG101"
                     /db_xref="taxon:1076"
     source          3081..5680
                     /organism="synthetic construct"
                     /mol_type="other DNA"
                     /label=derived from cloning vector pHSG298
                     /note="derived from cloning vector pHSG298"
                     /db_xref="taxon:32630"
     CDS             3331..4146
                     /codon_start=1
                     /product="aminoglucoside phosphotransferase"
                     /label=aminoglucoside phosphotransferase
                     /note="kanamycin-resistance"
                     /protein_id="BAA89407.1"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4147..4455)
                     /codon_start=1
                     /gene="lacZ alpha"
                     /label=lacZ alpha
                     /protein_id="BAA89408.1"
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGECDLFNKAAVPSSQRNALPVLQPINQF"
     gene            complement(4147..4455)
                     /gene="lacZ alpha"
                     /label=lacZ alpha
     primer_bind     4365..4381
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4385..4441
                     /label=multiple cloning sites
                     /note="multiple cloning sites"
     primer_bind     complement(4451..4467)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4475..4491
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4499..4529)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4544..4565
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5037..5625)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"