LOCUS       Exported                7052 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pMFXU4 DNA, complete sequence.
ACCESSION   AB072914
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7052)
  AUTHORS   Komeda T, Tazumi K, Shimada H, Kano K, Hyashi T, Saito H, Tsumura H,
            Sakai Y, Kato N, Kondo K.
  TITLE     Production of Active Bovine Cathepsin C (Dipeptidyl Aminopeptidase 
            I) in the Methylotrophic Yeast Candida boidinii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7052)
  AUTHORS   Komeda T, Kondo K, Sakai Y, Kato N.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2001) Toshihiro Komeda, Central Laboratories for 
            Key Technology, Kirin Brewery Co., Ltd.; 1-13-5, Fukuura, 
            Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan 
            (E-mail:t-komeda@kirin.co.jp, Tel:81-45-788-7217, 
            Fax:81-45-788-4042)
REFERENCE   3  (bases 1 to 7052)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7052
                     /organism="Expression vector pMFXU4"
                     /mol_type="other DNA"
                     /label=Candida boidinii expression vector
                     /note="Candida boidinii expression vector"
                     /db_xref="taxon:173630"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      425..1600
                     /regulatory_class="promoter"
                     /standard_name="mFDH1"
     misc_feature    1601
                     /label=NotI cloning site
                     /note="NotI cloning site"
     regulatory      1609..2237
                     /regulatory_class="terminator"
                     /standard_name="FDH1"
     CDS             complement(2891..3703)
                     /codon_start=1
                     /gene="URA3"
                     /product="orotidine-5-phosphate decarboxylase"
                     /label=URA3
                     /note="Candida boidinii orotidine-5-phosphate 
                     decarboxylase"
                     /protein_id="BAE72068.1"
                     /translation="MSETLKTQPYSKRAEVHPSPVARRLFNLMETKKTNLCASVDLTTT
                     KEILDLLEKVGPYICLVKTHIDIVSDFSYEGTIVPLLALAKKYNFMIFEDRKFADIGNT
                     VKSQYKGGVYQIAKWSDITNAHGVTGSGIVTGLKQAAEEVTDEPRGLLMLAELSSKGAI
                     AHGKYTEETVEIAKTDKDFVIGFIAQNQMGGSDEGFDWIIMTPGVGLDDKGDALGQQYR
                     TVSEVMSTGTDIIIVGRGLFGKGRDPSVEGERYMKAGWNAYLARTNQL"
     gene            complement(2891..3703)
                     /gene="URA3"
                     /label=URA3
     primer_bind     complement(4831..4847)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4855..4871
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4879..4909)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4924..4945
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5233..5821)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5992..6852)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6853..6957)
                     /gene="bla"
                     /label=AmpR promoter