LOCUS       Exported                9177 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pMF334, complete sequence.
ACCESSION   DQ250999
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9177)
  AUTHORS   Freitag M, Selker EU.
  TITLE     Expression and Visualization of Red Fluorescent Protein (RFP) in 
            Neurospora crassa
  JOURNAL   Fungal Genet. Newsl. 52 (2006) In press
REFERENCE   2  (bases 1 to 9177)
  AUTHORS   Freitag M, Selker EU.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2005) Institute of Molecular Biology, University 
            of Oregon, 355 Streisinger Hall, Eugene, OR 97403-1229, USA
REFERENCE   3  (bases 1 to 9177)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9177
                     /organism="Expression vector pMF334"
                     /lab_host="Neurospora crassa"
                     /mol_type="other DNA"
                     /label=for N-terminal fusions with ccg-1 promoter
                     /note="for N-terminal fusions with ccg-1 promoter"
                     /db_xref="taxon:355545"
     regulatory      complement(1..17)
                     /regulatory_class="promoter"
                     /note="SP6 promoter"
     promoter        363..467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             468..1328
                     /codon_start=1
                     /product="Bla"
                     /label=Bla
                     /note="beta-lactamase"
                     /protein_id="ABB59942.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1499..2087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      2345..2365
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(2379..4006)
                     /label=downstream flank of Neurospora crassa his-3 gene
                     /note="downstream flank of Neurospora crassa his-3 gene"
     CDS             2638..2649
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      4007..4027
                     /regulatory_class="promoter"
                     /note="T3 promoter"
     promoter        4008..4026
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      4051..4972
                     /regulatory_class="promoter"
                     /note="promoter of Neurospora crassa ccg-1 gene"
     CDS             4985..6376
                     /codon_start=1
                     /product="tdimer2(12)"
                     /label=tdimer2
                     /note="engineered dimer of Discosoma sp. dsRed for 
                     amino-terminal fusions; Reference: Campbell, R.E., Tour, 
                     O., Palmer, A.E., Steinbach, P.A., Baird, G.S., Zacharias, 
                     D.A., Tsien, R.Y., 2002. A monomeric red fluorescent 
                     protein. Proc. Nat. Acad. Sci. (USA) 99: 7877-7882; stop 
                     codon provided by insertion"
                     /protein_id="ABB59943.1"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQ
                     TAKLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFED
                     GGVVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGE
                     IHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEG
                     RHHLFL"
     misc_feature    6377..6388
                     /label=glycine linker
                     /note="glycine linker"
     misc_feature    6389..6464
                     /label=multiple cloning sites
                     /note="multiple cloning sites"
     regulatory      complement(6496..6516)
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     promoter        complement(6497..6515)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(6517..9162)
                     /note="truncated Neurospora crassa his-3 gene for gene 
                     replacements"
     promoter        complement(9176..17)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"