LOCUS       Exported                8122 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Neurospora expression vector pMF270, complete sequence.
ACCESSION   FJ456997
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8122)
  AUTHORS   Honda S, Selker EU.
  TITLE     Tools for fungal proteomics: multifunctional neurospora vectors for 
            gene replacement, protein expression and protein purification
  JOURNAL   Genetics 182 (1), 11-23 (2009)
  PUBMED    19171944
REFERENCE   2  (bases 1 to 8122)
  AUTHORS   Honda S, Selker EU.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2008) Institute of Molecular Biology, University 
            of Oregon, Eugene, OR 97403, USA
REFERENCE   3  (bases 1 to 8122)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8122
                     /organism="Neurospora expression vector pMF270"
                     /mol_type="other DNA"
                     /db_xref="taxon:591012"
     regulatory      complement(1..17)
                     /regulatory_class="promoter"
                     /note="SP6"
     promoter        363..467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             468..1328
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="ampicillin resistance"
                     /protein_id="ACM09997.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1499..2087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      complement(2345..2365)
                     /regulatory_class="promoter"
                     /note="T7"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(2374..4006)
                     /label=3 flank region of Neurospora crassa his-3 gene
                     /note="3 flank region of Neurospora crassa his-3 gene"
     CDS             2638..2649
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4008..4026
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4057..4985
                     /regulatory_class="promoter"
                     /note="Neurospora crassa ccg-1 (grg-1)"
     primer_bind     4981..4997
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5013..5120
                     /label=3xHA
                     /note="3xHA"
     CDS             5019..5108
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5141..5328
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    5376..5460
                     /note="T3 promoter and multiple cloning site from 
                     pBluescript KS+"
     primer_bind     complement(5396..5412)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(5442..5460)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    complement(6196..8103)
                     /note="5 truncated histidine biosynthesis trifunctional 
                     protein; includes phosphoribosyl-AMP cyclohydrolase, 
                     phosphoribosyl-ATP cyclohydrolase and histindinol 
                     dehydrogenase"
     promoter        complement(8121..17)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"