LOCUS       Exported                3858 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pMET7hIL-2Ralpha, complete sequence.
ACCESSION   LT727001
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 3858)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Mammalian expression vector pMET7hIL-2Ralpha"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 5280."
                     /db_xref="taxon:1945053"
     regulatory      9..632
                     /regulatory_class="promoter"
                     /note="SRa"
     promoter        18..632
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      198..333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      247..322
                     /label=SV40 ori
                     /note="SV40 ori"
     LTR             364..632
                     /label=5 LTR (truncated)
                     /note="truncated 5 long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          662..755
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     promoter        800..818
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      800..816
                     /regulatory_class="promoter"
                     /note="T7"
     misc_feature    861..923
                     /label=sIL2R
                     /note="sIL2R"
     CDS             924..1679
                     /codon_start=1
                     /note="unnamed protein product; hIL2R"
                     /protein_id="SJL87162.1"
                     /translation="ELCDDDPPEIPHATFKAMAYKEGTMLNCECKRGFRRIKSGSLYML
                     CTGNSSHSSWDNQCQCTSSATRNTTKQVTPQPEEQKERKTTEMQSPMQPVDQASLPGHC
                     REPPPWENEATERIYHFVVGQMVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQLIC
                     TGEMETSQFPGEEKPQASPEGRPESETSCLVTTTDFQIQTEMAATMETSIFTTEYQVAV
                     AGCVFLLISVLLLSGLTWQRRQRKSRRTI"
     regulatory      complement(1741..1837)
                     /regulatory_class="terminator"
                     /note="polyA"
     regulatory      1741..1837
                     /regulatory_class="terminator"
                     /note="polyA"
     polyA_signal    1763..1897
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1924..2325)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(2107..2695)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2866..3726)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL87163.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3727..3831)
                     /gene="bla"
                     /label=AmpR promoter