LOCUS       Exported                4501 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Bacterial selection and production vector pMES4, complete sequence.
ACCESSION   GQ907248
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Pellis M, Pardon E, Muyldermans S.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2009) Cellular and Molecular Immunology, VUB-VIB, 
            Pleinlaan 2, Brussels, Brussels 1050, Belgium
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Bacterial selection and production vector
                     pMES4"
                     /mol_type="other DNA"
                     /db_xref="taxon:680262"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     regulatory      143..198
                     /regulatory_class="promoter"
                     /note="pLac"
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      211..215
                     /regulatory_class="ribosome_binding_site"
     misc_feature    225..290
                     /label=encodes pelB signal peptide
                     /note="encodes pelB signal peptide"
     CDS             225..290
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     misc_feature    340..357
                     /label=His-tag
                     /note="His-tag"
     CDS             340..357
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    361..390
                     /label=HA-tag
                     /note="HA-tag"
     gene            391..1623
                     /gene="M13 geneIII"
                     /label=M13 geneIII
     primer_bind     complement(1630..1646)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1859..2314
                     /label=f1 ori
                     /note="f1 ori"
     promoter        2596..2700
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2701..3561
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="ampicillin resistance"
                     /protein_id="ACX42262.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3721..4335
                     /label=rep (pMB1)
                     /note="rep (pMB1)"
     rep_origin      3732..4320
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"