LOCUS       Exported                5303 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pME6040, complete sequence.
ACCESSION   AF133831
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Heeb S, Itoh Y, Nishijyo T, Schnider U, Keel C, Wade J, Walsh U, 
            OGara F, Haas D.
  TITLE     Small, stable shuttle vectors based on the minimal pVS1 replicon for
            use in gram-negative, plant-associated bacteria
  JOURNAL   Mol. Plant Microbe Interact. 13 (2), 232-237 (2000)
  PUBMED    10659714
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Heeb S.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-1999) Laboratoire de Biologie Microbienne, 
            University of Lausanne, Batiment de Biologie, Lausanne CH-1015, 
            Switzerland
REFERENCE   3  (bases 1 to 5303)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Shuttle vector pME6040"
                     /host="Pseudomonas pVS1; Escherichia p15A replicons"
                     /mol_type="other DNA"
                     /label=derived from plasmids pACYC177 and pME278
                     /note="derived from plasmids pACYC177 and pME278"
                     /db_xref="taxon:90149"
     CDS             195..824
                     /codon_start=1
                     /product="pVS1 partitioning protein"
                     /label=pVS1 partitioning protein
                     /note="StaA; similar to IncC2"
                     /protein_id="AAD21675.1"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             845..1060
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="ORF3; not required for stable maintenance"
                     /protein_id="AAD21674.1"
                     /translation="MSKSTNTLSAGRPSARSSKAATLASLADTPAMKRVNFQLPAEDHT
                     KLKMYAVRQGKTITELLSEYIAQLPE"
     misc_feature    1091..1103
                     /label=KorB box
                     /note="KorB box"
     CDS             1253..2326
                     /codon_start=1
                     /product="pVS1 replication protein"
                     /label=pVS1 replication protein
                     /note="RepA"
                     /protein_id="AAD21676.1"
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      2392..2586
                     /label=pVS1 origin of replication
                     /note="pVS1 origin of replication"
     misc_feature    2392..2399
                     /label=DnaA box
                     /note="DnaA box"
     repeat_region   2403..2486
                     /label=21 bp repeats
                     /note="21 bp repeats"
     misc_feature    2487..2578
                     /label=AT-rich region
                     /note="AT-rich region"
     misc_feature    2579..2586
                     /label=DnaA box
                     /note="DnaA box"
     rep_origin      2964..3793
                     /label=p15A origin of replication
                     /note="p15A origin of replication"
     rep_origin      2981..3526
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     misc_feature    3768..3789
                     /label=p15A origin of transfer
                     /note="p15A origin of transfer"
     misc_feature    3837..3947
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             4229..5044
                     /codon_start=1
                     /product="Tn903 aminoglycoside phosphotransferase"
                     /label=Tn903 aminoglycoside phosphotransferase
                     /note="Aph"
                     /protein_id="AAD21677.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"