LOCUS       Exported                3392 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pME18S-FL3-3 DNA, complete sequence.
ACCESSION   AB009864
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Maruyama K, Sugano S.
  TITLE     pME18S-FL3-3: a versatile expression vector
  JOURNAL   Published Only in Database (1997)
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Maruyama K, Sugano S.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-DEC-1997) Sumio Sugano, The Institute of Medical 
            Science, University of Tokyo, Department of Virology; 4-6-1, 
            Shirokanedai, Minatoku, Tokyo 108, Japan 
            (E-mail:ssugano@ims.u-tokyo.ac.jp, Tel:81-3-5449-5286, 
            Fax:81-3-5449-5416)
REFERENCE   3  (bases 1 to 3392)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On May 9, 2005 this sequence version replaced AB009864.1.
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Expression vector pME18S-FL3-3"
                     /plasmid="pME18S-FL3-3"
                     /mol_type="other DNA"
                     /db_xref="taxon:327037"
     regulatory      1..638
                     /regulatory_class="promoter"
     promoter        18..632
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      198..333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             364..632
                     /label=5 LTR (truncated)
                     /note="truncated 5 long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          662..755
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_site      1229..1427
     polyA_signal    1297..1431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1641..2229)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2400..3260)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3261..3365)
                     /gene="bla"
                     /label=AmpR promoter