LOCUS       Exported                3554 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pME-loxP-mCherry-pA-loxP, complete sequence.
ACCESSION   KF753698
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Sztal TE, Zhao M, Williams C, Oorschot V, Parslow AC, Giousoh A, 
            Yuen M, Hall TE, Costin A, Ramm G, Bird PI, Busch-Nentwich EM, 
            Stemple DL, Currie PD, Cooper ST, Laing NG, Nowak KJ, 
            Bryson-Richardson RJ.
  TITLE     Zebrafish models for nemaline myopathy reveal a spectrum of nemaline
            bodies contributing to reduced muscle function
  JOURNAL   Acta Neuropathol. 130 (3), 389-406 (2015)
  PUBMED    25931053
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Hall TE.
  TITLE     Cre-lox in zebrafish
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3554)
  AUTHORS   Hall TE.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2013) Institute for Molecular Bioscience, 
            University of Queensland, 306 Carmody Road, St Lucia, QLD 4067, 
            Australia
REFERENCE   4  (bases 1 to 3554)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Cloning vector pME-loxP-mCherry-pA-loxP"
                     /mol_type="other DNA"
                     /db_xref="taxon:1451182"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     536..553
                     /label=M13-fwd
                     /note="M13-fwd"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_recomb     570..665
                     /label=attL1
                     /note="attL1"
     misc_recomb     645..665
                     /label=attB1
                     /note="attB1"
     misc_recomb     669..702
                     /label=LoxP
                     /note="LoxP"
     protein_bind    669..702
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      703..712
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             709..1419
                     /codon_start=1
                     /product="mCherry"
                     /label=mCherry
                     /note="from Chien p3E with one mutation"
                     /protein_id="AHH01498.1"
                     /translation="MVSKGEDDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     promoter        complement(1435..1452)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1457..1591
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(1461..1644)
                     /label=SV40-polyA region
                     /note="SV40-polyA region"
     misc_recomb     1645..1678
                     /label=LoxP
                     /note="LoxP"
     protein_bind    1645..1678
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    complement(1679..1778)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_recomb     complement(1682..1777)
                     /label=attL2
                     /note="attL2"
     misc_recomb     complement(1682..1702)
                     /label=attB2
                     /note="attB2"
     primer_bind     complement(1795..1816)
                     /label=T7
                     /note="T7"
     promoter        complement(1796..1814)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1815..1835)
                     /label=M13-rev
                     /note="M13-rev"
     primer_bind     complement(1819..1835)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1948..2757
                     /codon_start=1
                     /product="Kan R+"
                     /label=Kan R+
                     /protein_id="AHH01497.1"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2900..3507
                     /label=pBR322 Origin
                     /note="pBR322 Origin"
     rep_origin      2904..3492
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"