LOCUS       Exported                3609 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pME-iCre, complete sequence.
ACCESSION   KF753697
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Sztal TE, Zhao M, Williams C, Oorschot V, Parslow AC, Giousoh A, 
            Yuen M, Hall TE, Costin A, Ramm G, Bird PI, Busch-Nentwich EM, 
            Stemple DL, Currie PD, Cooper ST, Laing NG, Nowak KJ, 
            Bryson-Richardson RJ.
  TITLE     Zebrafish models for nemaline myopathy reveal a spectrum of nemaline
            bodies contributing to reduced muscle function
  JOURNAL   Acta Neuropathol. 130 (3), 389-406 (2015)
  PUBMED    25931053
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Hall TE, Currie PD.
  TITLE     Cre-lox in zebrafish
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3609)
  AUTHORS   Hall TE, Currie PD.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2013) Institute for Molecular Bioscience, 
            University of Queensland, 306 Carmody Road, St Lucia, QLD 4067, 
            Australia
REFERENCE   4  (bases 1 to 3609)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Cloning vector pME-iCre"
                     /mol_type="other DNA"
                     /db_xref="taxon:1451181"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     536..553
                     /label=M13-fwd
                     /note="M13-fwd"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_recomb     570..665
                     /label=attL1
                     /note="attL1"
     misc_recomb     645..665
                     /label=attB1
                     /note="attB1"
     misc_feature    652..658
                     /label=Gateway recomb1
                     /note="Gateway recomb1"
     regulatory      671..680
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             683..703
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(1734..1833)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_recomb     complement(1737..1832)
                     /label=attL2
                     /note="attL2"
     misc_recomb     complement(1737..1757)
                     /label=attB2
                     /note="attB2"
     misc_feature    complement(1744..1750)
                     /label=Gateway recomb2
                     /note="Gateway recomb2"
     primer_bind     complement(1850..1871)
                     /label=T7
                     /note="T7"
     promoter        complement(1851..1869)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1870..1890)
                     /label=M13-rev
                     /note="M13-rev"
     primer_bind     complement(1874..1890)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2003..2812
                     /codon_start=1
                     /product="KanR"
                     /label=KanR
                     /protein_id="AHH01496.1"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2959..3547
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"