LOCUS       Exported                3311 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pME-Cavin1a-MO-Control, complete sequence.
ACCESSION   KM211533
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Lo HP, Nixon SJ, Hall TE, Cowling BS, Ferguson C, Morgan GP, 
            Schieber NL, Fernandez-Rojo MA, Bastiani M, Floetenmeyer M, Martel 
            N, Laporte J, Pilch PF, Parton RG.
  TITLE     The caveolin-cavin system plays a conserved and critical role in 
            mechanoprotection of skeletal muscle
  JOURNAL   J. Cell Biol. 210 (5), 833-849 (2015)
  PUBMED    26323694
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Lo H, Hall TE, Parton RG.
  TITLE     The role of Cavin1 in zebrafish
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3311)
  AUTHORS   Hall TE, Parton RG.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2014) Institute for Molecular Bioscience, 
            University of Queensland, 306 Carmody Road, St Lucia, QLD 4067, 
            Australia
REFERENCE   4  (bases 1 to 3311)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Cloning vector pME-Cavin1a-MO-Control"
                     /mol_type="other DNA"
                     /db_xref="taxon:1545817"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     536..553
                     /label=M13-fwd
                     /note="M13-fwd"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_recomb     570..665
                     /label=AttL1
                     /note="AttL1"
     misc_recomb     645..665
                     /label=AttB1
                     /note="AttB1"
     misc_feature    669..715
                     /label=zf Cavin1a 5 URS
                     /note="zf Cavin1a 5 URS"
     CDS             716..1435
                     /codon_start=1
                     /product="EGFP"
                     /label=EGFP
                     /protein_id="AIO08286.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    complement(1436..1535)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_recomb     complement(1439..1534)
                     /label=AttL2
                     /note="AttL2"
     misc_recomb     complement(1439..1459)
                     /label=AttB2
                     /note="AttB2"
     primer_bind     complement(1552..1573)
                     /label=T7
                     /note="T7"
     promoter        complement(1553..1571)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1572..1592)
                     /label=M13-rev
                     /note="M13-rev"
     primer_bind     complement(1576..1592)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1705..2514
                     /codon_start=1
                     /product="kanamycin resistance protein"
                     /label=kanamycin resistance protein
                     /note="Kan+"
                     /protein_id="AIO08285.1"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2657..3264
                     /label=pBR322 ori
                     /note="pBR322 ori"
     rep_origin      2661..3249
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"