LOCUS       Exported                6155 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Integration vector pMD-TV, complete sequence.
ACCESSION   JX436480
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Dharmasena MN, Hanisch BW, Wai TT, Kopecko DJ.
  TITLE     Stable expression of Shigella sonnei form I O-polysaccharide genes 
            recombineered into the chromosome of live Salmonella oral vaccine 
            vector Ty21a
  JOURNAL   Int. J. Med. Microbiol. 303 (3), 105-113 (2013)
  PUBMED    23474241
REFERENCE   2  (bases 1 to 6155)
  AUTHORS   Dharmasena MN, Hanisch BW, Wai TT, Kopecko DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2012) Laboratory of Enteric and Sexually 
            Transmitted Diseases, FDA-CBER, Lincoln Drive, Bethesda, MD 20892, 
            USA
REFERENCE   3  (bases 1 to 6155)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vertor NTI v. NTI
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6155
                     /organism="Integration vector pMD-TV"
                     /lab_host="Salmonella enterica subsp. enterica serovar 
                     Typhi"
                     /mol_type="other DNA"
                     /db_xref="taxon:1306879"
     CDS             109..1176
                     /codon_start=1
                     /gene="vexA"
                     /product="VexA"
                     /label=vexA
                     /protein_id="AGH89391.1"
                     /translation="MKKIIILLTTFFLLSGCTIPRAVFKSSLINQDDPRYNLVEVTPTL
                     KLSAPDTVPKTIVDPVFAANNWHWTSLAKGDVLHITILSSGGAGYLSNNASGDRADFEN
                     ILVTDSNTVQVPYAGTIPVSGLDVTQLADEIKKRLSRVVLNPQVIVTLTARTGAMVTVE
                     GSGKTGRYPLEQSMNRLSHLLATAVAVENTSTDMMEVHVTRQQHYFTARLSDIYQYPGL
                     DIALQPDDRITLRQVTEYVNVLGAAGVQGKHALVQRHSSVVDALALAKGLNDNLADPQA
                     IFLYKHNEAEQAKQQMRKLNIYHVDMSQPNSVFLAQAIRVDNGDVIYISNASLTDFAKV
                     KAAFDSFLTRGTNSF"
     gene            109..1176
                     /gene="vexA"
                     /label=vexA
     rep_origin      1658..1880
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             1928..2878
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     gene            3914..4420
                     /gene="tivD"
                     /label=tivD
     CDS             3914..4417
                     /codon_start=1
                     /gene="tivD"
                     /product="TivD"
                     /label=tivD
                     /protein_id="AGH89392.1"
                     /translation="PFLIDLIANVMSITLSFQNASMNKLFEKECRNVATRALKYVRQKK
                     TEGRLDEALSVLISLKRIEPDVSRLMREYKQIIRLFNESRKDGGSTITSYEHLDYAKKL
                     LVFDSENAYALKYAALNAMHLRDYTQALQYWQRLEKVNGPTEPVTRQISTCITALQKNT
                     SGKS"
     protein_bind    complement(4444..4491)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4852..5646
                     /codon_start=1
                     /gene="kanR"
                     /product="KanR"
                     /label=kanR
                     /protein_id="AGH89393.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            4852..5646
                     /gene="kanR"
                     /label=kanR
     protein_bind    complement(5837..5870)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"