LOCUS       Exported                4625 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pMCG50-S, complete sequence.
ACCESSION   AF053409
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Krieg AM, Wu T, Weeratna R, Efler SM, Love-Homan L, Yang L, Yi AK, 
            Short D, Davis HL.
  TITLE     Sequence motifs in adenoviral DNA block immune activation by 
            stimulatory CpG motifs
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 95 (21), 12631-12636 (1998)
  PUBMED    9770537
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Wu T, Efler SM, Davis HL, Krieg AM, Schorr J.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-1998) HGD, Loeb Health Research Institute, 725 
            Parkdale Ave., Ottawa, ON K1Y 4E9, Canada
REFERENCE   3  (bases 1 to 4625)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Expression vector pMCG50-S"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:82307"
     polyA_signal    77..284
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(312..328)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    336..352
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    407..428
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(716..1304)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1447..2256)
                     /codon_start=1
                     /gene="aph(3)-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3170..3186
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3193..3211
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3226..3242)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        3271..3650
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3651..3854
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             3926..4606
                     /codon_start=1
                     /gene="S"
                     /product="surface antigen subtype ayw"
                     /label=S
                     /note="derived from Hepatitis B virus surface antigen"
                     /protein_id="AAC64342.1"
                     /translation="MENITSGFLGPLLVLQAGFFLLTRILTIPQSLDSWWTSLNFLGGT
                     TVCLGQNSQSPTSNHSPTSCPPTCPGYRWMCLRRFIIFLFILLLCLIFLLVLLDYQGML
                     PVCPLIPGSSTTSTGPCRTCMTTAQGTSMYPSCCCTKPSDGNCTCIPIPSSWAFGKFLW
                     EWASARFSWLSLLVPFVQWFVGLSPTVWLSVIWMMWYWGPSLYSILSPFLPLLPIFFCL
                     WVYI"
     gene            3926..4606
                     /gene="S"
                     /label=S