LOCUS       Exported                7153 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Mycobacteria-E.coli shuttle probe vector pMC210, complete sequence.
ACCESSION   EU682406
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Fan XY, Ma H, Guo J, Li ZM, Cheng ZH, Guo SQ, Zhao GP.
  TITLE     A novel differential expression system for gene modulation in 
            Mycobacteria
  JOURNAL   Plasmid 61 (1), 39-46 (2009)
  PUBMED    18835406
REFERENCE   2  (bases 1 to 7153)
  AUTHORS   Fan X-Y., Li Z-M., Ma H, Guo J, Zhao G-P.
  TITLE     Analysis of promoter elements of Mycobacterium tuberculosis furA 
            gene in vivo and their application for recombinant BCG to induce 
            enhanced Th1 type immune responses
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7153)
  AUTHORS   Fan X-Y., Ma H, Guo J, Li Z-M., Zhao G-P.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2008) Department of Microbiology, Fudan 
            University, Shanghai 200433, China
REFERENCE   4  (bases 1 to 7153)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Mycobacteria-E.coli shuttle probe vector
                     pMC210"
                     /mol_type="other DNA"
                     /db_xref="taxon:529759"
     CDS             120..935
                     /codon_start=1
                     /gene="aph"
                     /product="aminoglycoside-3-phosphotransferase"
                     /label=aph
                     /note="kanamycin resistance; APH1 region of Tn903"
                     /protein_id="ACD68590.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            120..935
                     /gene="aph"
                     /label=aph
     rep_origin      1222..2009
                     /label=Escherichia coli OriE region derived from pUC19
                     /note="Escherichia coli OriE region derived from pUC19"
     rep_origin      1267..1855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2014..3909
                     /label=mycobacterial OriM region derived from pAL5000
                     /note="mycobacterial OriM region derived from pAL5000"
     misc_feature    3928..3971
                     /label=multicloning site
                     /note="multicloning site"
     primer_bind     3953..3969
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3971..7018
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /note="lacks first eight amino acids"
                     /protein_id="ACD68591.1"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            3971..7018
                     /gene="lacZ"
                     /label=lacZ
     terminator      7071..7117
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     regulatory      7074..7117
                     /regulatory_class="terminator"
                     /note="rrnBT1"