LOCUS       Exported                3967 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Moss gene targeting vector pMBL6attR, complete sequence.
ACCESSION   DQ228132
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Kamisugi Y, Cuming AC, Cove DJ.
  TITLE     Parameters determining the efficiency of gene targeting in the moss 
            Physcomitrella patens
  JOURNAL   Nucleic Acids Res. 33 (19), E173 (2005)
  PUBMED    16282584
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Kamisugi Y, Cove DJ, Cuming AC.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-OCT-2005) Centre for Plant Sciences, Leeds University,
            Leeds, West Yorkshire LS2 9JT, UK
REFERENCE   3  (bases 1 to 3967)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Moss gene targeting vector pMBL6attR"
                     /lab_host="Physcomitrella patens"
                     /mol_type="other DNA"
                     /db_xref="taxon:351700"
     CDS             394..699
                     /codon_start=1
                     /gene="ccdB"
                     /product="ccdB"
                     /label=ccdB
                     /note="Gateway conversion cassette; interferes with DNA 
                     gyrase"
                     /protein_id="ABB17068.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            394..699
                     /gene="ccdB"
                     /label=ccdB
     misc_recomb     740..864
                     /note="AttR2 site; Gateway conversion cassette"
     protein_bind    complement(740..864)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(923..939)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    947..963
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(971..1001)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1016..1037
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1325..1913)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2084..2944)
                     /codon_start=1
                     /gene="bla"
                     /product="beta lactamase"
                     /function="confers resistance to beta-lactam antibiotics"
                     /label=bla
                     /protein_id="ABB17067.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2084..2944)
                     /gene="bla"
                     /label=bla
     promoter        complement(2945..3049)
                     /gene="bla"
                     /label=AmpR promoter
     misc_recomb     3127..3251
                     /note="AttR1 site; Gateway conversion cassette"
     protein_bind    3127..3251
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3276..3306
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3360..52
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyl transferase"
                     /function="chloramphenicol resistance"
                     /label=cat
                     /note="Gateway conversion cassette"
                     /protein_id="ABB17069.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            3360..52
                     /gene="cat"
                     /label=cat