LOCUS       Exported                4296 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Moss transformation vector pMBL5, complete sequence.
ACCESSION   DQ228130
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Knight CD, Cove DJ, Cuming AC, Quatrano RS.
  TITLE     Moss Gene Technology
  JOURNAL   (in) Gilmartin,P.M. and Bowler,C. (Eds.); MOLECULAR PLANT BIOLOGY: 
            285-301; Oxford University Press, Oxford, NY, USA (2002)
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Machuka J, Knight CD, Cove DJ, Cuming AC.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2005) Centre for Plant Sciences, Leeds University,
            Leeds, West Yorkshire LS2 9JT, UK
REFERENCE   3  (bases 1 to 4296)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Moss transformation vector pMBL5"
                     /lab_host="Physcomitrella patens"
                     /mol_type="other DNA"
                     /db_xref="taxon:351698"
     primer_bind     complement(10..26)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    34..50
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(58..88)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    103..124
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(412..1000)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1171..2031)
                     /codon_start=1
                     /gene="bla"
                     /product="beta lactamase"
                     /function="confers resistance to beta-lactam antibiotics"
                     /label=bla
                     /protein_id="ABB17063.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(1171..2031)
                     /gene="bla"
                     /label=bla
     promoter        complement(2032..2136)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    2181..2288
                     /label=pBluescriptII SK+ multiple cloning site
                     /note="pBluescriptII SK+ multiple cloning site"
     primer_bind     2205..2221
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2255..2271)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2290..2680
                     /regulatory_class="promoter"
                     /note="Cauliflower Mosaic Virus 35S promoter"
     promoter        2338..2683
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2713..3507
                     /codon_start=1
                     /gene="nptII"
                     /product="neomycin phosphotransferase"
                     /function="confers resistance to kanamycin and G418"
                     /label=nptII
                     /protein_id="ABB17064.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            2713..3507
                     /gene="nptII"
                     /label=nptII
     polyA_signal    3576..3752
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     regulatory      3609..4260
                     /regulatory_class="terminator"
                     /note="Cauliflower Mosaic Virus gene 6 terminator"