LOCUS       Exported                5655 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMB1.MjYRS, complete sequence.
ACCESSION   KU055484
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Tack DS, Ellefson JW, Thyer R, Wang B, Gollihar J, Forster MT, 
            Ellington AD.
  TITLE     Addicting diverse bacteria to a non-canonical amino acid
  JOURNAL   Nat. Chem. Biol. (2016) In press
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Tack DS, Ellefson JW, Thyer R, Wang B, Gollihar J, Forster MT, 
            Ellington AD.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2015) Molecular Biosciences, University of Texas, 
            2500 Speedway, Austin, TX 78660, USA
REFERENCE   3  (bases 1 to 5655)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Cloning vector pMB1.MjYRS"
                     /mol_type="other DNA"
                     /label=MjYRS-Nap3
                     /note="MjYRS-Nap3"
                     /db_xref="taxon:1776437"
     CDS             1221..2231
                     /codon_start=1
                     /gene="aadA"
                     /product="streptomycin 3-adenylyltransferase"
                     /EC_number="2.7.7.47"
                     /label=aadA
                     /note="spectinomycin and streptomycin resistance protein"
                     /protein_id="ALV85906.1"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFAHYVKGEITKVVGK"
     gene            1221..2231
                     /gene="aadA"
                     /label=aadA
     CDS             1440..2231
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFAHYVKGEITKVVGK"
     promoter        complement(2342..2444)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      2668..3515
                     /gene="p15A"
     gene            2668..3515
                     /gene="p15A"
                     /label=p15A
     rep_origin      complement(2970..3515)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     gene            3653..4734
                     /gene="tyrS"
                     /label=tyrS
                     /note="ptyrS"
     regulatory      3653..3800
                     /regulatory_class="promoter"
                     /gene="tyrS"
     CDS             3814..4734
                     /codon_start=1
                     /gene="tyrS"
                     /product="tyrosyl-tRNA synthetase"
                     /EC_number="6.1.1.1"
                     /label=tyrS
                     /note="tyrosine-tRNA ligase; derived from 
                     Methanocaldococcus jannaschii; charges amber suppressing 
                     tRNA-Nap3"
                     /protein_id="ALV85905.1"
                     /translation="MDEFEMIKRNTSEIISEEELREVLKKDEKSAYIGFEPSGKIHLGH
                     YLQIKKMIDLQNAGFDIIILLADLHAYLNQKGELDEIRKIGDYNKKVFEAMGLKAKYVY
                     GSEFQLDKDYTLNVYRLALKTTLKRARRSMELIAREDENPKVAEVIYPIMQVNDIHYLG
                     VDVAVGGMEQRKIHMLARELLPKKVVCIHNPVLTGLDGEGKMSSSKGNFIAVDDSPEEI
                     RAKIKKAYCPAGVVEGNPIMEIAKYFLEYPLTIKRPEKFGGDLTVNSYEELESLFKNKE
                     LHPMDLKNAVAEELIKILEPIRKRL"
     regulatory      4911..4959
                     /regulatory_class="promoter"
                     /gene="lpp"
     gene            4911..4959
                     /gene="lpp"
                     /label=lpp
     tRNA            4977..5053
                     /product="tRNA-Tyr"
                     /label=3-iodo-tyrosine non-canonical amino acid
                     /note="3-iodo-tyrosine non-canonical amino acid"
     regulatory      5078..5107
                     /regulatory_class="terminator"
                     /gene="rrnB"
     gene            5078..5107
                     /gene="rrnB"
                     /label=rrnB