LOCUS       Exported                5742 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Episomal vector pMARS-mODC-AZ, complete sequence.
ACCESSION   EU421131
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Kitsera N, Khobta A, Epe B.
  TITLE     Destabilized green fluorescent protein detects rapid removal of 
            transcription blocks after genotoxic exposure
  JOURNAL   BioTechniques 43 (2), 222-227 (2007)
  PUBMED    17824390
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Khobta A, Kitsera N, Epe B.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2008) Institute of Pharmacy, Johannes Gutenberg 
            University of Mainz, Staudingerweg 5, Mainz 55099, Germany
REFERENCE   3  (bases 1 to 5742)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Episomal vector pMARS-mODC-AZ"
                     /mol_type="other DNA"
                     /db_xref="taxon:502370"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      607..616
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             613..1485
                     /codon_start=1
                     /gene="EGFP-mODC"
                     /product="EGFP-mODC"
                     /label=EGFP-mODC
                     /note="destabilised enhanced green fluorecent protein; EGFP
                     is destabilized by addition of a fragment encoding for 
                     mouse ornithine decarboxylase (mODC) 
                     proline-glutamate-serine-threonine-rich (PEST) sequence; 
                     fusion EGFP-mODC protein is efficiently degraded by 
                     proteasomes (protein half-life in mammalian cells of 
                     approximately 30 minutes)"
                     /protein_id="ABZ91087.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKSGLRSLMKQIQSHGFPPEVEEQDDGTLPMSCAQESGMDRHPA
                     ACASARINV"
     gene            613..1485
                     /gene="EGFP-mODC"
                     /label=EGFP-mODC
     CDS             613..1329
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    2372..2493
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2500..2955)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2982..3086
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3306..3567
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             3638..4432
                     /codon_start=1
                     /product="neomycin phosphotransferase"
                     /label=neomycin phosphotransferase
                     /protein_id="ABZ91088.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4664..4711
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5040..5628
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"