LOCUS       Exported                7538 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pmAlbEPintPlauGH DNA, complete sequence.
ACCESSION   AB453180
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Suemizu H, Hasegawa M, Kawai K, Taniguchi K, Monnai M, Wakui M, 
            Suematsu M, Ito M, Peltz G, Nakamura M.
  TITLE     Establishment of a humanized model of liver using NOD/Shi-scid 
            IL2Rgnull mice
  JOURNAL   Biochem. Biophys. Res. Commun. 377 (1), 248-252 (2008)
  PUBMED    18840406
REFERENCE   2  (bases 1 to 7538)
  AUTHORS   Suemizu H, Hasegawa M, Kawai K, Taniguchi K, Monnai M, Wakui M, 
            Suematsu M, Ito M, Peltz G, Nakamura M.
  TITLE     Expression vector pmAlbEPintPlauGH DNA, complete sequence
  JOURNAL   Published Only in Database (2008)
REFERENCE   3  (bases 1 to 7538)
  AUTHORS   Suemizu H, Hasegawa M, Kawai K, Nakamura M.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-2008) Contact:Hiroshi Suemizu Central Institute 
            for Experimental Animals, Biomedical Research Department; 3-25-12 
            Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-0821, Japan
REFERENCE   4  (bases 1 to 7538)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7538
                     /organism="Mammalian expression vector pmAlbEPintPlauGH"
                     /mol_type="other DNA"
                     /note="Expression vector derived from pBluescript II KS(+),
                     Stratagene Corp., La Jolla, CA"
                     /db_xref="taxon:553144"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     misc_feature    135..441
                     /label=Phage f1 region
                     /note="Phage f1 region"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      669..3020
                     /regulatory_class="promoter"
                     /note="mouse Albumin enhancer/promoter region"
     intron          3130..3262
                     /note="chimeric intron derived from pCI, Promega Corp., 
                     Madison, WI"
     promoter        3307..3325
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3340..4641
                     /codon_start=1
                     /gene="Plau"
                     /product="urokinase-type plasminogen activator"
                     /label=Plau
                     /protein_id="BAH02680.1"
                     /translation="MKVWLASLFLCALVVKNSEGGSVLGAPDESNCGCQNGGVCVSYKY
                     FSRIRRCSCPRKFQGEHCEIDASKTCYHGNGDSYRGKANTDTKGRPCLAWNAPAVLQKP
                     YNAHRPDAISLGLGKHNYCRNPDNQKRPWCYVQIGLRQFVQECMVHDCSLSKKPSSSVD
                     QQGFQCGQKALRPRFKIVGGEFTEVENQPWFAAIYQKNKGGSPPSFKCGGSLISPCWVA
                     SAAHCFIQLPKKENYVVYLGQSKESSYNPGEMKFEVEQLILHEYYREDSLAYHNDIALL
                     KIRTSTGQCAQPSRSIQTICLPPRFTDAPFGSDCEITGFGKESESDYLYPKNLKMSVVK
                     LVSHEQCMQPHYYGSEINYKMLCAADPEWKTDSCKGDSGGPLICNIEGRPTLSGIVSWG
                     RGCAEKNKPGVYTRVSHFLDWIQSHIGEEKGLAF"
     gene            3340..4641
                     /gene="Plau"
                     /label=Plau
     CDS             4495..4506
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    4691..5317
                     /note="DNA fragment contains poly A signal, human growth 
                     hormone 3-flanking sequence"
     polyA_signal    4694..5316
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     promoter        complement(5350..5368)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5389..5405)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5413..5429
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5437..5467)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5482..5503
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    5735..6402
                     /label=pUC plasmid replication origin
                     /note="pUC plasmid replication origin"
     rep_origin      complement(5791..6379)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6550..7410)
                     /codon_start=1
                     /gene="AmpR"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="ampicillin resistance protein"
                     /protein_id="BAH02681.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(6550..7410)
                     /gene="AmpR"
                     /label=AmpR
     promoter        complement(7411..7515)
                     /gene="bla"
                     /label=AmpR promoter