LOCUS       Exported                3836 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMAK27, complete sequence.
ACCESSION   FJ040214
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Koronfel MA.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2008) Crop Science, Faculty of Agriculture - Cairo
            University, Cairo University Street, Giza 12613, Egypt
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Cloning vector pMAK27"
                     /mol_type="other DNA"
                     /db_xref="taxon:555091"
     CDS             1..366
                     /codon_start=1
                     /product="lacZ alpha-peptide"
                     /label=lacZ alpha-peptide
                     /protein_id="ACH69348.1"
                     /translation="MTMITPSSNTTHYRESSVPRMLQTRYVSDPEFVDKLLEPRLALDH
                     TCGGPSSRPLYSIVSPKWPHNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWKL"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      24..40
                     /regulatory_class="promoter"
                     /note="T7"
     misc_feature    51..163
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        complement(169..187)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      170..187
                     /regulatory_class="promoter"
                     /note="SP6"
     primer_bind     complement(201..217)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(358..813)
                     /direction=LEFT
                     /label=phage f1
                     /note="phage f1"
     promoter        840..944
                     /gene="bla"
                     /label=AmpR promoter
     CDS             945..1805
                     /codon_start=1
                     /gene="bla"
                     /product="ampicillin resistance protein"
                     /label=bla
                     /protein_id="ACH69349.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            945..1805
                     /gene="bla"
                     /label=bla
     CDS             1951..2766
                     /codon_start=1
                     /gene="kan"
                     /product="kanamycin resistance protein"
                     /label=kan
                     /note="neomycin phosphotransferase"
                     /protein_id="ACH69350.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            1951..2766
                     /gene="kan"
                     /label=kan
     rep_origin      2851..3439
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      3438
                     /label=pUC
                     /note="pUC"
     protein_bind    3727..3748
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3763..3793
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3801..3817
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3825..5
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"