LOCUS       Exported                9670 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMAD2, complete sequence.
ACCESSION   KT323982
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9670)
  AUTHORS   Bonnin RA, Le Lam TN, Bohn C, Bouloc P.
  TITLE     Deciphering RNase III essentiality in Staphylococcus aureus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9670)
  AUTHORS   Bonnin RA, Le Lam TN, Bohn C, Bouloc P.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUL-2015) Institut de Biologie Integrative de la 
            Cellule, Faculte Paris Sud, Batiment 400, Orsay 91400, France
REFERENCE   3  (bases 1 to 9670)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9670
                     /organism="Cloning vector pMAD2"
                     /mol_type="other DNA"
                     /db_xref="taxon:1883451"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(300..1034)
                     /codon_start=1
                     /gene="ermC"
                     /product="ErmC"
                     /label=ermC
                     /note="erythromycin resistance protein"
                     /protein_id="ANZ22902.1"
                     /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFT
                     LELVKRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIYGNIPY
                     NISTDIIRKIVFDSIANEIYLIVEYGFAKRLLNTKRSLALLLMAEVDISILSMVPREYF
                     HPKPKVNSSLIRLSRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGID
                     DLNNISFEQFLSLFNSYKLFNK"
     gene            complement(300..1034)
                     /gene="ermC"
                     /label=ermC
     CDS             1446..2657
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /note="replicase"
                     /protein_id="ANZ22903.1"
                     /translation="MSHSILRVARVKGSSNTNGIQRHNQRENKNYNNKDINHEETYKNY
                     DLINAQNIKYKDKIDETIDENYSGKRKIRSDAIRHVDGLVTSDKDFFDDLSGEEIERFF
                     KDSLEFLENEYGKENMLYATVHLDERVPHMHFGFVPLTEDGRLSAKEQLGNKKDFTQLQ
                     DRFNEYVNEKGYELERGTSKEVTEREHKAMDQYKKDTVFHKQELQEVKDELQKANKQLQ
                     SGIEHMRSTKPFDYENERTGLFSGREETGRKILTADEFERLQETISSAERIVDDYENIK
                     STDYYTENQELKKRRESLKEVVNTWKEGYHEKSKEVNKLKRENDSLNEQLNVSEKFQDS
                     TVTLYRAARANFPGFEKGFNRLKEKFFNDSKFERVGQFMDVVQDNVQKVDRKREKQRTD
                     DLEM"
     gene            1446..2657
                     /gene="repA"
                     /label=repA
     CDS             complement(4176..6194)
                     /codon_start=1
                     /gene="bgaB"
                     /product="BgaB"
                     /label=bgaB
                     /note="beta-galactosidase"
                     /protein_id="ANZ22904.1"
                     /translation="MNVLSSICYGGDYNPEQWPEEIWYEDAKLMQKAGVNLVSLGIFSW
                     SKIEPSDGVFDFEWLDKVIDILYDHGVYINLGTATATTPAWFVKKYPDSLPIDESGVIL
                     SFGSRQHYCPNHPQLITHIKRLVRAIAERYKNHPALKMWHVNNEYACHVSKCFCENCAV
                     AFRKWLKERYKTIDELNERWGTNFWGQRYNHWDEINPPRKAPTFINPSQELDYYRFMND
                     SILKLFLTEKEILREVTPDIPVSTNFMGSFKPLNYFQWAQHVDIVTWDSYPDPREGLPI
                     QHAMMNDLMRSLRKGQPFILMEQVTSHVNWRDINVPKPPGVMRLWSYATIARGADGIMF
                     FQWRQSRAGAEKFHGAMVPHFLNENNRIYREVTQLGQELKKLDCLVGSRIKAEVAIIFD
                     WENWWAVELSSKPHNKLRYIPIVEAYYRELYKRNIAVDFVRPSDDLTKYKVVIAPMLYM
                     VKEGEDENLRQFVANGGTLIVSFFSGIVDENDRVHLGGYPGPLRDILGIFVEEFVPYPE
                     TKVNKIYSNDGEYDCTTWADIIRLEGAEPLATFKGDWYAGLPAVTRNCYGKGEGIYVGT
                     YPDSNYLGRLLEQVFAKHHINPILEVAENVEVQQRETDEWKYLIIINHNDYEVTLSLPE
                     DKIYQNMIDGKCFRGGELRIQGVDVAVLREHDEAGKV"
     gene            complement(4176..6194)
                     /gene="bgaB"
                     /label=bgaB
     CDS             7432..7623
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    7725..7865
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8051..8639)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8810..9670)
                     /codon_start=1
                     /gene="blaTEM-1"
                     /product="beta-lactamase TEM-1"
                     /label=blaTEM-1
                     /note="ampicillin resistance protein"
                     /protein_id="ANZ22905.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(8810..9670)
                     /gene="blaTEM-1"
                     /label=blaTEM-1