LOCUS       Exported                4861 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMAB143, complete sequence.
ACCESSION   DQ062691
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Stewart DIH., Wiersma EJ, Druar C, Saini SS, Cossitt MA.
  TITLE     Analysis of the expressed repertoire of Macaca fascicularis V heavy 
            genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Stewart DIH., Wiersma EJ, Druar C, Saini SS, Cossitt MA.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2005) Cangene Corporation, 3403 American Dr., 
            Mississauga, ON L4V 1T4, Canada
REFERENCE   3  (bases 1 to 4861)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Cloning vector pMAB143"
                     /mol_type="other DNA"
                     /db_xref="taxon:329480"
     misc_feature    34..93
                     /label=second pel B leader
                     /note="second pel B leader"
     misc_feature    108..421
                     /label=first constant domain of Homo sapiens IgG1
                     /note="first constant domain of Homo sapiens IgG1"
     misc_feature    433..1647
                     /note="coat protein derived from phage fd; gene 3; lacks 5
                     end"
     misc_feature    1657..1674
                     /label=hexahistidine tag
                     /note="hexahistidine tag"
     CDS             1657..1674
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      1685..1713
                     /regulatory_class="terminator"
     primer_bind     complement(1719..1735)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1948..2403
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2685..2789
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2790..3650
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="ampicillin resistance"
                     /protein_id="AAY46370.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3821..4409
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      4685..4806
                     /regulatory_class="promoter"
                     /note="lac operon and promoter"
     protein_bind    4697..4718
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4733..4763
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4771..4787
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    4807..11
                     /label=first pelB leader
                     /note="first pelB leader"
     CDS             4807..11
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"