LOCUS       Exported                6740 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMAB136, complete sequence.
ACCESSION   DQ119053
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Soltes G, Hust M, Ng KK, Bansal A, Field J, Stewart DI, Dubel S, Cha
            S, Wiersma EJ.
  TITLE     On the influence of vector design on antibody phage display
  JOURNAL   J. Biotechnol. 127 (4), 626-637 (2007)
  PUBMED    16996161
REFERENCE   2  (bases 1 to 6740)
  AUTHORS   Soltes GA, EngBerg J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2005) Biology, Cangene Corporation, 3403 American 
            Drive, Mississauga, ON L4V1T4, Canada
REFERENCE   3  (bases 1 to 6740)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Cloning vector pMAB136"
                     /mol_type="other DNA"
                     /note="E. coli pBR322/M13 phage vector for display of human
                     antibody genes"
                     /db_xref="taxon:339959"
     regulatory      569..686
                     /regulatory_class="promoter"
                     /note="lac promoter operator"
     protein_bind    577..598
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        613..643
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    651..667
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             687..1394
                     /codon_start=1
                     /product="pelB/anti-tetanus toxoid immunoglobulin kappa 
                     light chain variable region fusion protein precursor"
                     /label=pelB/anti-tetanus toxoid immunoglobulin kappa l
                     /protein_id="AAZ78137.1"
                     /translation="MKYLLPTAAAGLLLLAAQPAMADIQMTQSPSSLSASVGDRVIITC
                     RASQSISTYLNWYQQKPGKAPKLLIYYATNLQSGVPSRFSGSGSGTDFTLTISSLQPED
                     FATYYCQQSSNTVTFGPGTKVDMKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPR
                     EAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGL
                     SSPVTKSFNRGEC"
     sig_peptide     687..752
                     /note="pelB signal peptide"
     CDS             687..752
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     mat_peptide     753..1391
                     /product="anti-tetanus toxoid immunoglobulin kappa light 
                     chain variable region"
                     /note="derived from Homo sapiens"
     CDS             1419..3401
                     /codon_start=1
                     /product="pelB/anti-tetanus toxoid immunoglobulin gamma 
                     heavy chain/gIIIp fusion protein precursor"
                     /label=pelB/anti-tetanus toxoid immunoglobulin gamma h
                     /protein_id="AAZ78138.1"
                     /translation="MKYLLPTAAAGLLLLAAQPAIAQVQLVQSGGGLVQPGGSVRLSCA
                     ASGFSFSSYAMSWVRQAPGMGLEWVAAISARGTTTYYADSVTGRLTISRDNSMNTLYLH
                     LNSLRAEDTAVYYCAKAGKQWLAHYYFDSWGQGTLVTVSSASTKGPSVFPLAPSSKSTS
                     GGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGT
                     QTYICNVNHKPSNTKVDKKAEPKSCAAAGSKDIRTVESCLAKPHTENSFTNVWKDDKTL
                     DRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGG
                     TKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQ
                     GALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQ
                     GQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKM
                     ANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGS
                     NSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPYVFGAGKPYEFSIDCDKINLFRGVF
                     AFLLYVATFMYVFSTFANILRNKES"
     sig_peptide     1419..1484
                     /note="pelB signal peptide"
     misc_feature    1485..1853
                     /note="derived from Homo sapiens; Region: IgG1 VH1 specific
                     for tetanus toxoid"
     misc_feature    1854..2159
                     /note="derived from Homo sapiens; Region: IgG1 CH1 domain"
     misc_feature    2187..3398
                     /note="derived from M13 phage; Region: gIIIp"
     CDS             3453..3470
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(3495..3513)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3520..3536)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3681..4136
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4165..4269
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4270..5130
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5301..5889
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        6080..6157
                     /gene="lacI"
                     /label=lacI promoter