LOCUS       Exported                9713 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Binary cloning vector pMAA-Red, complete sequence.
ACCESSION   JX025642
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9713)
  AUTHORS   Ali MA, Shah KH, Bohlmann H.
  TITLE     pMAA-Red: Binary cloning vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9713)
  AUTHORS   Ali MA, Shah KH, Bohlmann H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2012) Department of Crop Sciences, Division of 
            Plant Protection, University of Natural Resources and Life Sciences,
            Konrad Lorenz Strasse 24, Tulln, Lower Austria 3430, Austria
REFERENCE   3  (bases 1 to 9713)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9713
                     /organism="Binary cloning vector pMAA-Red"
                     /mol_type="other DNA"
                     /db_xref="taxon:1200950"
     CDS             404..1033
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             1462..2535
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QATADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      2601..2795
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     rep_origin      complement(3133..3752)
                     /direction=LEFT
                     /note="bom region; derived from pBR322"
     rep_origin      complement(3148..3736)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3822..4616)
                     /codon_start=1
                     /gene="kan1"
                     /product="Kan1"
                     /function="kanamycin resistance"
                     /label=kan1
                     /protein_id="AFM74423.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(3822..4616)
                     /gene="kan1"
                     /label=kan1
     misc_feature    5045..5069
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      5138..5530
                     /regulatory_class="promoter"
                     /note="PDF2.1 from Arabidopsis plant defensin 2.1"
     CDS             5531..6208
                     /codon_start=1
                     /gene="dsred2"
                     /product="DsRed2"
                     /label=dsred2
                     /note="reporter florescent protein from Discosoma sp."
                     /protein_id="AFM74424.1"
                     /translation="MTSSENV??KFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     gene            5531..6208
                     /gene="dsred2"
                     /label=dsred2
     regulatory      6209..6464
                     /regulatory_class="terminator"
                     /note="nopaline synthase (nos)"
     terminator      6209..6461
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6465..6500
                     /note="polylinker; multiple cloning site"
     regulatory      6501..7415
                     /regulatory_class="promoter"
                     /note="CamV.2x35S+Omega"
     promoter        6992..7337
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    7357..7412
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5-leader sequence (Gallie et al., 1988)"
     CDS             7416..9227
                     /codon_start=1
                     /gene="uidA"
                     /product="UidA"
                     /label=uidA
                     /note="GUS reporter"
                     /protein_id="AFM74425.1"
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            7416..9227
                     /gene="uidA"
                     /label=uidA
     regulatory      9279..9518
                     /regulatory_class="terminator"
                     /note="CaMV_35S"
     misc_feature    9575..9599
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"