LOCUS       Exported                4075 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Dual pho-lac fusion vector pMA632, complete sequence.
ACCESSION   AF087958
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Alexeyev MF, Winkler HH.
  TITLE     Membrane topology of the Rickettsia prowazekii ATP/ADP translocase 
            revealed by novel dual pho-lac reporters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Alexeyev MF.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-1998) Microbiology and Immunology, University of 
            South Alabama, LMB Building, Mobile, AL 36688-0001, USA
REFERENCE   3  (bases 1 to 4075)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Dual pho-lac fusion vector pMA632"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:83672"
     rep_origin      complement(3..459)
                     /direction=LEFT
                     /label=f1 origin of replication
                     /note="f1 origin of replication"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     misc_feature    532..551
                     /label=polylinker
                     /note="polylinker"
     misc_feature    560..2065
                     /note="represents translational fusion between truncated 
                     alkaline phosphatase and beta-galactosidase alpha fragment;
                     5 terminus of the alkaline phosphatase gene truncated in 
                     order to make it useful as a topological reporter"
     primer_bind     complement(1909..1925)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2070..2089
                     /label=polylinker
                     /note="polylinker"
     rep_origin      complement(2146..3086)
                     /direction=LEFT
                     /label=ColE1 origin of replication
                     /note="ColE1 origin of replication"
     rep_origin      complement(2328..2916)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3087..3947)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="confers resistance to ampicillin"
                     /label=bla
                     /protein_id="AAC72101.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3087..3947)
                     /gene="bla"
                     /label=bla
     promoter        complement(3948..4052)
                     /gene="bla"
                     /label=AmpR promoter