LOCUS       Exported               10508 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pLX-WMoV, complete sequence.
ACCESSION   MH200603
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10508)
  AUTHORS   Pasin F, Tseng XA, Bedoya LC, Heydarnejad J, Deng TC, Garcia JA, 
            Chen YR.
  TITLE     Streamlined generation of plant virus infectious clones using the 
            pLX mini binary vectors
  JOURNAL   J. Virol. Methods (2018) In press
  PUBMED    30236898
REFERENCE   2  (bases 1 to 10508)
  AUTHORS   Pasin F.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2018) Agricultural Biotechnology Research Center, 
            Academia Sinica, 128 Academia Road, Section 2, Nangang District, 
            Taipei, Taipei 11529, Taiwan
REFERENCE   3  (bases 1 to 10508)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: UltraCycler v. v1.0
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10508
                     /organism="Binary vector pLX-WMoV"
                     /mol_type="other DNA"
                     /db_xref="taxon:2364173"
     regulatory      25..72
                     /regulatory_class="terminator"
                     /note="bacterial terminator"
     misc_feature    133..157
                     /label=LB
                     /note="LB"
     misc_feature    180..210
                     /label=LB
                     /note="LB"
     misc_feature    184..208
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      260..1203
                     /regulatory_class="promoter"
                     /note="CaMV 35S promoter"
     promoter        1158..1203
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    1204..7500
                     /label=Wasabi mottle virus
                     /note="Wasabi mottle virus"
     CDS             1269..6062
                     /codon_start=1
                     /transl_except="(pos: 4578 .. 4580,  aa: OTHER)"
                     /product="RNA replicase readthrough protein"
                     /label=RNA replicase readthrough protein
                     /protein_id="AYC81452.1"
                     /translation="MAQFQQTIDMQTLQAAAGRNSLVNDLASRRVYDNAVEELNARSRR
                     PKVHFSKSVSTEQTLLASNAYPEFEISFTHTQHAVHSLAGGLRSLELEYLMMQVPFGSL
                     TYDIGGNFAAHLFKGRDYVHCCMPNLDVRDIARHEGHKEAVHGYISRLKRQRRPVPEYQ
                     RAAFNNYSENPHFVHCDQPFQQCALSTVNGDDTYAIALHSVYDIPVEEFGGALLRKNVK
                     VCYAAFHFHENMLLDCDTVTLEEIGATFQRAGDKLNFFFHNESTLNYTHSFSNIIKYVC
                     KTFFPASQRFVYHKEFLVTRVNTWYCKFTRVDTFTLFRGVYKNSVDSDEFYKAMDDAWE
                     YKKTLAMLNAERTIFKDNAAINFWFPKVRDMVIVPLFDASITTGRMSRREVMVNKDFVY
                     TVLNHIKTYQAKALTYANVLSFVESIRSRVIINGVTARSEWDTDKAILGPLAMTFFLVT
                     KLGHVQDEIVLKKFQKFDSTAKELIWTSLCDALMGVIPSVKETLARGGFVKLAEESLEI
                     KIPELYCTFTDRLVLEYKRAEEFKSCDLSKPLEESEKYYNALSELSVLENLDSFDLEAF
                     KELCQKKSVDPDVAAKVVVAIMKSELTLPFKKPTEEEIAESLSNDTIRNEGLSLSNTAP
                     FPCVSNLEGGLVPACGLCPKSGGFDKIDMDISEFHLMSVDAVKKGAMMSAVYTGNIKVQ
                     QMKNFVDYLSASLSATVSNLCKVLRDVHGVDPESQEKSGVWDVRRGRWLLKPNAKCHAW
                     GVAEDANHKLVIVLLNWDDGKPVCDQTWYRLAVSSDSLVYSDIGKLKTLTSCCVDGEPP
                     EPKAKVVLVDGVPGCGKTKEILEKVNFSEDLVLVPGKEASKMIIRRANQAGVTRADRDN
                     VRTVDSFLMHPPKRVFKRLFIDEGLMLHTGCVNFLTLISQCDIAYVYGDTQQIPFICRV
                     ANFPYPKHFARLVVDEKEDRRITLRCPADVTFFLNKKYDGSVLCTSSVERSVSAQVVRG
                     KGALNPITLPLEGKILTFTQADKFELLDKGYKDVNTVHEVQGETYEKTAIVRLTATPLE
                     IISRASPHVLVALTRHTTSCKYFSVVLDPLVSVISEMEKLSNFILDMYKVESGTQ*QLQ
                     IDAVFKGVNLNVPTPKSGDWRDMQFYYDTLLPGNSTILNEFDAVTMNLRDISLNVKDCR
                     IDFSKSVQVPKEKPVFLKPKIRTAAEMPRTAGLIENLVAMIKRNMNAPDLTGTIDIEDT
                     ASLVVEKFWDSYIIKEFSGIEGMARTRQGFSRWLSKQESSTVGQLADFNFVDLPAVDEY
                     KHMIKSQPKQKLDLSIQDEYPALQTIVYHSKKINAIFGPMFSELTRMLLERVDTSKFLF
                     YTRKTPTQIEEFFSDLDSSQAMEILELDISKYDKSQNEFHCAVEYKIWEKLGIDEWLAE
                     VWRQGHRKTTLKDYTAGIKTCLWYQRKSGDVTTFIGNTIIIAACLSSMIPMEKVIKAAF
                     CGDDSLIYIPKGLDLPDIQAGANLTWNFEAKLFRKKYGYFCGRYVIHHDRGAIVYYDPL
                     KLISKLGCKHIKDEVHLEELRRSLCDVASNLNNCAYFSQLDEAVAEVHKTAVGGSFAYC
                     SIIKYLSDKRLFKDLFFV"
     CDS             1269..4580
                     /codon_start=1
                     /product="RNA replicase"
                     /label=RNA replicase
                     /protein_id="AYC81451.1"
                     /translation="MAQFQQTIDMQTLQAAAGRNSLVNDLASRRVYDNAVEELNARSRR
                     PKVHFSKSVSTEQTLLASNAYPEFEISFTHTQHAVHSLAGGLRSLELEYLMMQVPFGSL
                     TYDIGGNFAAHLFKGRDYVHCCMPNLDVRDIARHEGHKEAVHGYISRLKRQRRPVPEYQ
                     RAAFNNYSENPHFVHCDQPFQQCALSTVNGDDTYAIALHSVYDIPVEEFGGALLRKNVK
                     VCYAAFHFHENMLLDCDTVTLEEIGATFQRAGDKLNFFFHNESTLNYTHSFSNIIKYVC
                     KTFFPASQRFVYHKEFLVTRVNTWYCKFTRVDTFTLFRGVYKNSVDSDEFYKAMDDAWE
                     YKKTLAMLNAERTIFKDNAAINFWFPKVRDMVIVPLFDASITTGRMSRREVMVNKDFVY
                     TVLNHIKTYQAKALTYANVLSFVESIRSRVIINGVTARSEWDTDKAILGPLAMTFFLVT
                     KLGHVQDEIVLKKFQKFDSTAKELIWTSLCDALMGVIPSVKETLARGGFVKLAEESLEI
                     KIPELYCTFTDRLVLEYKRAEEFKSCDLSKPLEESEKYYNALSELSVLENLDSFDLEAF
                     KELCQKKSVDPDVAAKVVVAIMKSELTLPFKKPTEEEIAESLSNDTIRNEGLSLSNTAP
                     FPCVSNLEGGLVPACGLCPKSGGFDKIDMDISEFHLMSVDAVKKGAMMSAVYTGNIKVQ
                     QMKNFVDYLSASLSATVSNLCKVLRDVHGVDPESQEKSGVWDVRRGRWLLKPNAKCHAW
                     GVAEDANHKLVIVLLNWDDGKPVCDQTWYRLAVSSDSLVYSDIGKLKTLTSCCVDGEPP
                     EPKAKVVLVDGVPGCGKTKEILEKVNFSEDLVLVPGKEASKMIIRRANQAGVTRADRDN
                     VRTVDSFLMHPPKRVFKRLFIDEGLMLHTGCVNFLTLISQCDIAYVYGDTQQIPFICRV
                     ANFPYPKHFARLVVDEKEDRRITLRCPADVTFFLNKKYDGSVLCTSSVERSVSAQVVRG
                     KGALNPITLPLEGKILTFTQADKFELLDKGYKDVNTVHEVQGETYEKTAIVRLTATPLE
                     IISRASPHVLVALTRHTTSCKYFSVVLDPLVSVISEMEKLSNFILDMYKVESGTQ"
     CDS             6065..6868
                     /codon_start=1
                     /product="movement protein"
                     /label=movement protein
                     /protein_id="AYC81453.1"
                     /translation="MSTVSYKPKVSDFLALSRTEEILPKALTRLKTVSISTKDVISVKE
                     SESLCDIDLLVNVPLDKFRYVGILGVVFTGEWLIPDFVKGGVTVSVIDKRLENSKECII
                     GTYRAAAKDRRFQFKLVPNYFVSVTDAKRKPWQVHVRIQNLKIEAGWQPLALEVVSVAM
                     VTNNVVVKGLRERVIAVDDPNVEGFEGVVDEFIDSVAAFKAVDNFRKKKRKIGGRDVIS
                     KYKYRPEKYAGPDSLLNKEENVIQHHELESVPVFRSGMGGAHSNA"
     CDS             6792..7265
                     /codon_start=1
                     /product="coat protein"
                     /label=coat protein
                     /protein_id="AYC81454.1"
                     /translation="MSYNITNSNQYQFFAAVWAEPIAMLNQCVSALSQSYQTQAARDTV
                     RQQFSNLLSAIVTPNQRFPETGYRVYVNSAVLKPLYEALMKSFDTKNRIIETEEESRPS
                     ASEVANATQRVDDATVAIRSQIQLLLSELSSGHGLMNRAEFEVLIPWATAPAK"
     misc_feature    7501..7546
                     /label=ribozyme
                     /note="ribozyme"
     regulatory      7554..7809
                     /regulatory_class="terminator"
                     /note="nos terminator"
     terminator      7557..7809
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    7874..7897
                     /label=RB
                     /note="RB"
     regulatory      7962..8002
                     /regulatory_class="terminator"
                     /note="bacterial terminator"
     CDS             complement(8046..8861)
                     /codon_start=1
                     /product="kanamycin resistance protein"
                     /label=kanamycin resistance protein
                     /note="NptI"
                     /protein_id="AYC81455.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8987..9649)
                     /codon_start=1
                     /product="replication protein"
                     /label=replication protein
                     /protein_id="AYC81456.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      9650..10421
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"