LOCUS       Exported               13212 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLX-UCBSV, complete sequence.
ACCESSION   KY825157
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez 
            D, Garcia JA.
  TITLE     Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors 
            with Compatible Replication Origins
  JOURNAL   ACS Synth Biol (2017) In press
  PUBMED    28657330
REFERENCE   2  (bases 1 to 13212)
  AUTHORS   Pasin F, Garcia JA.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2017) Department of Plant Molecular Genetics, 
            Centro Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 13212)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Cloning vector pLX-UCBSV"
                     /mol_type="other DNA"
                     /db_xref="taxon:2016279"
     misc_feature    1..24
                     /label=assembly linker
                     /note="assembly linker"
     regulatory      25..72
                     /regulatory_class="terminator"
                     /note="artificial; tested only in sense orientation"
     misc_feature    133..157
                     /label=LB
                     /note="LB"
     misc_feature    180..210
                     /label=LB
                     /note="LB"
     misc_feature    184..208
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      440..1182
                     /regulatory_class="promoter"
                     /note="2x CaMV 35S"
     promoter        841..1185
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    1183..10275
                     /note="DSMZ: PV-0912; Ugandan cassava brown streak virus 
                     isolate Ke_125"
     CDS             1317..10025
                     /codon_start=1
                     /product="polyprotein"
                     /label=polyprotein
                     /protein_id="ASG92166.1"
                     /translation="MSTIQLFKTIRFGSFEPVKLDEGNNIIEKVPVDLLAGNDGSGPEE
                     QSEQKYHRKESGESWRKVTDLYSVIGNSVYCRSYVAMKNFLNDTKWGGLFKNKKGQELK
                     AAARLRRATSYGFMYDPVACAFECPVCRTKATALEAFTSDCDHCFEIKHIDDDRIVQTE
                     TKFYPINPIELDVEDNLVEAASLEWLKGDVEERVVDRVLLLEDKEIHVTKKALVKRRAE
                     TKLVANAADLTKKLTEICCESGIPIIDIDNSKRKAIPMVKLKHIFGKVECDDMFEEDRY
                     FLEHSNAGKIFRSCEKITYRMIRPGWSGAIILRDNVQSEDHDKFDFINDICVVQGKNLI
                     SYKIENAMRVKTETELDLIDLYSFNLSWAKSKDKFIKHFESDTTQLVRSCCTPSTLWLY
                     ARKARFYKFVDHMILKGSPIIDILVKMEYVGKHLDMFNSVEDVCTEYSHFMKELISETV
                     NDKSDPDVLRVRNLIRAHFESVLEYNKYELIDRIIEKKTQLEAQEIMSRELIKHQYGEL
                     FSWRERLCLKLGIGSSNLYTYWVEREEKQSEKSNVISCIISKPGIEMLINWVSEVCKSK
                     YHSLVNCVDSGILFLWSRIVHLARVSVYGYWNLWFRQAMCVLFIFLVSNFSGKIVSYLK
                     KLIVSEKKLAIKNEEGFVEVQGRKEESFVLKWCAAATLFLSFLNYDWAVGCVSAIGKMK
                     TIFSALGPDFIEKQDGDDDLKFTTFEVEIPGDSRSSSAQTFGEWIEHCIKFNLVSIEPT
                     TSGPMLTLERGKANELADQINCLNATDIRVHGGVGTGKSTALPYELIRYGAVLVCVPTR
                     VLANALHESFMSLFGFDVSLAYRGRVRTGSKPITIMTYGYALNHFHHNPRNLAQFQFII
                     MDEVHTFPVHLNPLFSLLRELSPDKKIIKTSATHVGHSVDLSTNHKVDIHTLEIMDVKK
                     WAELQGTSVFGDVTKEPGNVLVFVASYRDVDVCSDKLKDKGFPVIKVDGRNFRKNTEVQ
                     KMVDGMQGEVKFIVATNIIENGVTLDVDVVVDFGERISPNLCSEDRCILMQRQRISQAE
                     RKQRFGRVGRMKRGSVYKFGRETLPDSMRNRVGSTESALICFAYGLKPVVDDVDIGSVR
                     SVTQRQALTASMFEANYIFTAHLVDKQGFMPRPVFELMKSLLLHTDAVGVSSTYLATNM
                     SGWRRLKEYIRIDDNSRHVQEVQIPWYCSDMSDDFIVKLTECVKAAKPKSQCGYKVDNV
                     DFHTVAHKISVGESNIDESRALVATILDEVKQWRDGITYHSSTPRNKSLMSLMVGWIPR
                     KAEKTKEILDNRIQRLELLLNQLNGVRGIDDYESLVRFFSENPHSAEYLESQCASDYIE
                     EKVMSVKRNYDKSLILGMVGLAVATGTFAYWYMRRSAAVELVEKQAKHKYNRDKRTGRL
                     MFDMDDRETYENFGPEYTDDVISAKMTKAQKERDSKKKGWKAGKINRPMRVFHQLYGVN
                     PLEFDEVVMRVGKLETEPWDVKELNVDAMMIELDDDYHILRDDRMFGKKVSLAFKKEGA
                     DEETIVNLTPHRSKMTSSMSLAPMGFPEEEGRWRQTGAPLIRKIEKEDEVEVQVAKPES
                     TNPYDHILVRLGRAHLGTRVLNCFFHGSKCVIPYHLAEKGDREESLVIATTRGQFDFGP
                     MKNIKCRKVTDYDITICPLPNDVQPFRSKIVMREPKLGEEVVIVCFTRINGKIVMKVSD
                     KSTTYPAGGQFAHLWAYKYDGQPGDCGGPIVATVDQKVVGFHSGVIRNSREEKLRAVYT
                     PVNQELLNCISGDIQMTDFWTFNPDLVEWNSVARVSTFFPMTKAINTITVQANEGEELI
                     DGNLMIVGYVNREVYHNHVIKGKRESFMRYCEQFPNCAFTKELRDQYLPSILSKPAFRK
                     GLLKYNEPVRVGSVNFSCLIRAYLKVEEMFEDLGFLEEAGPQWDPIEILDDLNKKAAMG
                     ALYQGKKQDWLKSIEPADFITAVRESFKHLAGGDVGIWSGSLKAELRPVEKVLEQKTRV
                     FTGAPIDLLLGGKILVDNFNHFFYFNHLKGPWTVGINKFNKGWDRLARYFNHSWNFIDC
                     DGSRFDTSLAPILFQLVCHMREKFGNFDDIERAALRNLYTQIVYTPILTIDGYITKKHR
                     GNNSGQPSTVVDNTIILMIVVEYCKAVMESEGRVMQFKYMCNGDDLILNVPDDEVSIVQ
                     SRFRELFSECGLDYNFDDVHKSIETIEYMSHSFMLKDGVYIPKLKKERIVAILEWERGD
                     EIMRTRSALNAAYIESYGYDDLMVEIERYAVFWATDKGCEYPLLDRKRVEGLYKDDYTD
                     INEEWLIGILPPSFEHCYVDTQTKDLRGREKLELRIESHDRTLQMQMKFPVTFVTGNLG
                     KLAEVKSILGIANDVMAKNIDLPEVQGTPEEIVRKKAQLAVKMTNSPVLVEDTCLCFNA
                     FNGLPGPYIKWFLKELGLDGVVKMLSAFGDKSAYALCTFAYVHNESSDPIVFKGVVNGE
                     IVPPRGNNGFGWDPIFKPDGCGCTFAEMPSSIKNDFSHRRRALEKVKLFLDNLMVKQEK
                     NKASVALTIDVQALNQEEIEAEITALKKLWKDNGPTRTRSSFEARRLRAPQVERVNELL
                     QKLKDEGLQTKKRPCGEPDDGEVVDDDSNDGDNQRSEKEVVDESQNNQQVDPRKLKFKI
                     RGDGNAIRRDDIDKIPTNALEIKKTFKPPKVSQSAYIWIPRSQRDNLTPDVIQNFLAYI
                     PPSHAIDNQLASGIEVENWAIEVAKAYGVNIQEFYRTVLPAWIVNCIVNGTSDERKNEK
                     SWRAVELNSQGEDVDDFEYPMEPMYKFALPTMRKVMRNFSSQAILMYQNSVAAGKAFVI
                     KAARNAGYTSIENKWLGIDFLAEAQLSQSQLDIKHQILAANVGRSKTRLFALAAPGDDG
                     NVDKERHTTHDVSANRHSYSGAAIE"
     mat_peptide     1317..2402
                     /product="P1"
     mat_peptide     2403..3284
                     /product="P3"
     mat_peptide     3285..3440
                     /product="6K1"
     mat_peptide     3441..5324
                     /product="CI"
     mat_peptide     5325..5480
                     /product="6K2"
     mat_peptide     5481..6035
                     /product="VPg"
     mat_peptide     6036..6737
                     /product="NIa-Pro"
     mat_peptide     6738..8243
                     /product="NIb"
     mat_peptide     8244..8921
                     /product="Ham 1-like protein"
     mat_peptide     8922..10022
                     /product="coat protein"
     regulatory      10278..10535
                     /regulatory_class="terminator"
                     /note="nos"
     terminator      10288..10540
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    10578..10601
                     /label=RB
                     /note="RB"
     regulatory      complement(10666..10706)
                     /regulatory_class="terminator"
     misc_feature    10716..10739
                     /label=assembly linker
                     /note="assembly linker"
     CDS             complement(10750..11565)
                     /codon_start=1
                     /gene="neo"
                     /product="neomycin phosphotransferase"
                     /label=neo
                     /note="kanamycin resistance protein; NptI"
                     /protein_id="ASG92164.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(10750..11565)
                     /gene="neo"
                     /label=neo
     misc_feature    11667..11690
                     /label=assembly linker
                     /note="assembly linker"
     CDS             complement(11691..12353)
                     /codon_start=1
                     /gene="rep"
                     /product="replication protein"
                     /label=rep
                     /protein_id="ASG92165.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     gene            complement(11691..12353)
                     /gene="rep"
                     /label=rep
     rep_origin      12354..13125
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"