LOCUS       Exported               14423 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pLX-TuMV, complete sequence.
ACCESSION   MH200604
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14423)
  AUTHORS   Pasin F, Tseng XA, Bedoya LC, Heydarnejad J, Deng TC, Garcia JA, 
            Chen YR.
  TITLE     Streamlined generation of plant virus infectious clones using the 
            pLX mini binary vectors
  JOURNAL   J. Virol. Methods (2018) In press
  PUBMED    30236898
REFERENCE   2  (bases 1 to 14423)
  AUTHORS   Pasin F.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2018) Agricultural Biotechnology Research Center, 
            Academia Sinica, 128 Academia Road, Section 2, Nangang District, 
            Taipei, Taipei 11529, Taiwan
REFERENCE   3  (bases 1 to 14423)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: UltraCycler v. v1.0
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14423
                     /organism="Binary vector pLX-TuMV"
                     /mol_type="other DNA"
                     /db_xref="taxon:2364172"
     regulatory      25..72
                     /regulatory_class="terminator"
                     /note="bacterial terminator"
     misc_feature    133..157
                     /label=LB
                     /note="LB"
     misc_feature    180..210
                     /label=LB
                     /note="LB"
     misc_feature    184..208
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      440..856
                     /regulatory_class="promoter"
                     /note="CaMV 35S promoter"
     promoter        514..859
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    857..11428
                     /label=Turnip mosaic virus UK1, DAE, GFP
                     /note="Turnip mosaic virus UK1, DAE, GFP"
     CDS             987..11219
                     /codon_start=1
                     /product="polyprotein"
                     /label=polyprotein
                     /protein_id="AYC81457.1"
                     /translation="MAAVTFASAITNAITSKPALTGMVQFGSFPPMPLRSTTVTTVATS
                     VAQPKLYTVQFGSLDPVVVKSGAGSLAKATRQQPNVEIDVSLSEAAALEVAKPRSNAVL
                     RMHEEANKERALFLDWEASLKRSSYGIAEDEKVVMTTHGVSKIVPRSSRAMKLKRARER
                     RRAQQPIILKWEPKLSGISIGGGLSASVIEAEEVRTKWPLHKTPSMKKRTVHRICKMND
                     QGVDMLTRSLVKIFKTKSANIEYIGKKSIKVDFIRKERTKFARIQVAHLLGKRAQRDLL
                     TGMEENHFIDILSKYSGNKTTINPGVVCAGWSGIVVGNGILTQKRSRSPSEAFVIRGEH
                     EGKLYDARIKVTRTMSHKIVHFSAAGANFWKGFDRCFLAYRSDNREHTCYSGLDVTECG
                     EVAALMCLAMFPCGKITCPDCVTDSELSQGQASGPSMKHRLTQLRDVIKSSYPRFKHAV
                     QILDRYEQSLSSANENYQDFAEIQSISDGVEKAAFPHVNKLNAILIKGATVTGEEFSQA
                     TKHLLEIARYLKNRTENIEKGSLKSFRNKISQKAHINPTLMCDNQLDRNGNFIWGERGY
                     HAKRFFSNYFEIIDPKKGYTQYETRAVPNGSRKLAIGKLIVPTNFEVLREQMKGEPVEP
                     YPVTVECVSKLQGDFVHACCCVTTESGDPVLSEIKMPTKHHLVIGNSGDPKYIDLPEIE
                     ENKMYIAKEGYCYINIFLAMLVNVKESQAKEFTKVVRDKLVGELGKWPTLLDVATACYF
                     LKVFYPDVANAELPRMLVDHKTKIIHVVDSYGSLSTGYHVLKTNTVEQLIKFTRCNLES
                     SLKHYRVGGTEWEDTHGSSNIDNPQWCIKRLIKGVYKPKQLKEDMLANPFLPLYALLSP
                     GVILAFYNSGSLEYLMNHYIRVDSNVAVLLVVLKSLAKKVSTSQSVLAQLQIIERSLPE
                     LIEAKANVNGPDDAATRACNRFMGMLLHMAEPNWELADGGYTILRDHSISILEKSYLQI
                     LDEAWNELSWSERCAIRYYSSKQAIFTQKDLPMKSEADLGGRYSVSVMSSYERSKQCMK
                     SVHSSIGNRLRSSMSWTSSKVSNSVCRTINYLVPDVFKFMNVLVCISLLIKMTAEANHI
                     VTTQRRLKLDVEETERRKIEWELAFHHAILTQSAGQHPTIDEFRAYIADKAPHLSEHIE
                     PEEKAVVHQAKRQSEQELERIIAFVALVLMMFDAERSDCVTKILNKLKGLVATVEPTVY
                     HQTLNDIEDDLSERNLFVDFELSSDGDMLQQLPAEKTFASWWSHQLSRGFTIPHYRTEG
                     KFMTFTRATATEVAGKIAHESDKDILLMGAVGSGKSTGLPYHLSRKGNVLLLEPTRPLA
                     ENVHKQLSQAPFHQNTTLRMRGLTAFGSAPISVMTSGFALNYFANNRMRIEEFDFVIFD
                     ECHVHDANAMAMRCLLHECDYSGKIIKVSATPPGREVEFSTQYPVSISTEDTLSFQDFV
                     NAQGSGSNCDVISKGDNILVYVASYNEVDALSKLLIERDFKVTKVDGRTMKVGNIEITT
                     SGTPSKKHFIVATNIIENGVTLDIDVVADFGTKVLPYLDTDSRMLSTTKTSINYGERIQ
                     RLGRVGRHKPGHALRIGHTEKGLSEVPSCIATEAALKCFTYGLPVITNNVSTSILGNVT
                     VKQARTMSVFEITPFYTSQVVRYDGSMHPQVHALLKRFKLRDSEIVLNKLAIPHRGVNA
                     WLTASEYARLGANVEDRRDVRIPFMCRDIPEKLHLDMWDVIVKFKGDAGFGRLSSASAS
                     KVAYTLQTDVNSIQRTVTIIDTLIAEERRKQEYFKTVTSNCVSSSNFSLQSITNAIKSR
                     MMKDHTCENISVLEGAKSQLLEFRNLNADHSFATKTDGISRHFMSEYGALEAVHHQNTS
                     DMSKFLKLKGKWNKTLITRDVLVLCGVLGGGLWMVIQHLRSKMSEPVTHEAKGKRQRQK
                     LKFRNARDNKMGREVYGDDDTIEHFFGDAYTKKGKSKGRTRGIGHKNRKFINMYGFDPE
                     DFSAVRFVDPLTGATLDDNPLTDITLVQEHFGNIRMDLLGEDELDSNEIRVNKTIQAYY
                     MNNKTGKALKVDLTPHIPLKVCDLHATIAGFPERENELRQTGKAQPINIDEVPRANNEL
                     VPVDHESNSMFRGLRDYNPISNNICHLTNVSDGASNSLYGVGFGPLILTNRHLFERNNG
                     ELVIKSRHGEFVIKNTTQLHLLPIPDRDLLLIRLPKDVPPFPQKLGFRQPEKGERICMV
                     GSNFQTKSITSIVSETSTIMPVENSQFWKHWISTKDGQCGSPMVSTKDGKILGLHSLAN
                     FQNSINYFAAFPDDFAEKYLHTIEAHEWVKHWKYNTSAISWGSLNIQASQPSGLFKVSK
                     LISDLDSTAVYAQTQQNRWMFEQLNGNLKAIAHCPSQLVTKHTVKGKCQMFDLYLKLHD
                     EAREYFQPMLGQYQKSKLNREAYAKDLLKYATPIEAGNIDCDLFEKTVEIVVSDLRGYG
                     FETCNYVTDENDIFEALNMKSAVGALYKGKKKDYFAEFTPEMKEEILKQSCERLFLGKM
                     GVWNGSLKAELRPLEKVEANKTRTFTAAPLDTLLGGKVCVDDFNNQFYDHNLRAPWSVG
                     MTKFYCGWDRLLESLPDGWVYCDADGSQFDSSLSPYLINAVLNIRLGFMEEWDIGEVML
                     RNLYTEIVYTPISTPDGTLVKKFKGNNSGQPSTVVDNTLMVILAVNYSLKKSGIPSELR
                     DSIIRFFVNGDDLLLSVHPEYEYILDTMADNFRELGLKYTFDSRTREKGDLWFMSHQGH
                     KREGIWIPKLEPERIVSILEWDRSKEPCHRLEAICAAMIESWGYDKLTHEIRKFYAWMI
                     EQAPFSSLAQEGKAPYIAETALRKLYLDKEPAQEDLTHYLQAIFEDYEDGAEACVYHQA
                     GMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWP
                     TLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGD
                     TLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSV
                     QLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDE
                     LYKACVYHQAGETLDAELTDEQKQAEKEKKEREKAEKERERQKQLALKKGKDVAQEEGK
                     RDKEVNAGTSGTFSVPRLKSLTSKMRVPRYEKRVALNLDHLILYTPEQTDLSNTRSTRK
                     QFDTWFEGVMADYELTEDKMRIILNGLMVWCIENGTSPNINGMWVMMDGDDQVEFPIKP
                     LIDHAKPTFRQIMAHFSDVAEAYIEKRNQDRPYMPRYGLQRNLTDMSLARYAFDFYEMT
                     SRTPIRAREAHIQMKAAALRGANNNLFGLDGNVGTTVENTERHTTEDVNRNMHNLLGVQ
                     GL"
     misc_feature    9615..10352
                     /label=smGFP
                     /note="smGFP"
     regulatory      11496..11751
                     /regulatory_class="terminator"
                     /note="nos terminator"
     terminator      11499..11751
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    11789..11812
                     /label=RB
                     /note="RB"
     regulatory      11877..11917
                     /regulatory_class="terminator"
                     /note="bacterial terminator"
     CDS             complement(11961..12776)
                     /codon_start=1
                     /product="kanamycin resistance protein"
                     /label=kanamycin resistance protein
                     /note="NptI"
                     /protein_id="AYC81458.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(12902..13564)
                     /codon_start=1
                     /product="replication protein"
                     /label=replication protein
                     /protein_id="AYC81459.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      13565..14336
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"