LOCUS       Exported                7625 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pLX-TCTV, complete sequence.
ACCESSION   MH200605
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Pasin F, Tseng XA, Bedoya LC, Heydarnejad J, Deng TC, Garcia JA, 
            Chen YR.
  TITLE     Streamlined generation of plant virus infectious clones using the 
            pLX mini binary vectors
  JOURNAL   J. Virol. Methods (2018) In press
  PUBMED    30236898
REFERENCE   2  (bases 1 to 7625)
  AUTHORS   Pasin F.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2018) Agricultural Biotechnology Research Center, 
            Academia Sinica, 128 Academia Road, Section 2, Nangang District, 
            Taipei, Taipei 11529, Taiwan
REFERENCE   3  (bases 1 to 7625)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: UltraCycler v. v1.0
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Binary vector pLX-TCTV"
                     /mol_type="other DNA"
                     /db_xref="taxon:2364171"
     regulatory      25..72
                     /regulatory_class="terminator"
                     /note="bacterial terminator"
     misc_feature    133..157
                     /label=LB
                     /note="LB"
     misc_feature    180..210
                     /label=LB
                     /note="LB"
     misc_feature    184..208
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      260..1203
                     /regulatory_class="promoter"
                     /note="CaMV 35S promoter"
     promoter        1158..1203
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    1204..4671
                     /note="Turnip curly top virus isolate IR:Zaf:B11:06 
                     (1.16x); GU456685"
     CDS             1578..2693
                     /codon_start=1
                     /product="Rep"
                     /label=Rep
                     /protein_id="AYC81460.1"
                     /translation="MPRTPRQYRIHAKNIFLTYPHCHLTKEDALLQLQTIQCPSTKKFI
                     RICRERHDNGEPHLHVLIQFEGKIQLYNPRHFDLRDGGSGRICHPNIQGAKSSSDVKSY
                     IEKDGDYIDWGEFQIDARSARGGQQTANDACAEALNSGTAEAALAVIREKLPKDYIFQF
                     HNLKPNLAAIFNPPPVGYVPKYNHTQFVLTDDILDWLESNFFLEESNSPAGPSKYKVIP
                     SIDRPKSIIIEGPSRTGKTLWARSLGSHNYITGHLDFSTKVYNDDVSYNVIDDVDPHYL
                     KMKHWKHLIGAQKEWQTNLKYGKPRIIKGGIPSIILCNPGDGASYQNFLDKPENEALKS
                     WSLQNSVFTTIEEPLFNITSDQEVEDSTPTV"
     CDS             1738..1995
                     /codon_start=1
                     /product="C4"
                     /label=C4
                     /protein_id="AYC81461.1"
                     /translation="MGNLISMCLSNSKEKSSSITPVISIYEMEVPVASATQIFRELNRA
                     PTSSPISRRTEITSTGVNFRSMHDLHEEVNRRLMMHVQRR"
     CDS             2581..3006
                     /codon_start=1
                     /product="C2"
                     /label=C2
                     /protein_id="AYC81462.1"
                     /translation="MKPLSPGVYKIQSSQQSRNLSSISRVIKKSKTVLLPCKCKFTIHH
                     ECGEGFTHRGTHNSSSINDWNIHTSSTKPVSSQTNIMGPPGERVLQHQITTPIQLQPTE
                     TTGITHGLDRIHGIGSSPEFDWTSIYDDFFEEITLLP"
     CDS             2714..3136
                     /codon_start=1
                     /product="C3"
                     /label=C3
                     /protein_id="AYC81463.1"
                     /translation="MSVVRDLRTGEPITHRQSTIGTYIHQVPNPFHLKLISWDHQENGF
                     FNIRLQLRFNYNLRKQLGLHMAWIEFTVLGRHRSLTGHRFTTIFLKRLLFYLNNLGIIS
                     LNLVINGISHVLFKDFKFVESVINQEYRVAMKEEFY"
     CDS             complement(3133..3963)
                     /codon_start=1
                     /product="coat protein"
                     /label=coat protein
                     /protein_id="AYC81464.1"
                     /translation="MSGTWSLKRPRWHSPGYDLAPKTPVKRPAVRRALFNNQAQFSRVA
                     ARRRWSKFPVRGNKPYRYRKLKASDYQIYKDKIGGDNGWTVTFSGDCTMLNNYVRGIGR
                     DQRDSVMTKTAHMHFNGVLMANDAFWEAPNYMTMYSWIILDNDPGGTFPKPSDIFDMEY
                     KEFPSMYEVAESVKSRFIVKRKTEHYLRSTGVAFGEKQNYKAPNVGPVKKPIRMKFRNI
                     WQPSEWKDTAGGKYEDLKKGALLYVCICDNKATQFSFNLKGQWTMYFINRDLMY"
     CDS             complement(3818..4195)
                     /codon_start=1
                     /product="V2"
                     /label=V2
                     /protein_id="AYC81465.1"
                     /translation="MIWRIAKDDKRVRAFRYKKRRFYSLSEYLLAPLATHLRLARILPE
                     KCWSVVPAYTDEAYSFLFMLKEETRTPFQILKDEWNVEFKEAEVALSRLRLSTEDTGEE
                     TGGEESPVQQSSPVQSSSCET"
     regulatory      4671..4926
                     /regulatory_class="terminator"
                     /note="nos terminator"
     terminator      4674..4926
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4991..5014
                     /label=RB
                     /note="RB"
     regulatory      5079..5119
                     /regulatory_class="terminator"
                     /note="bacterial terminator"
     CDS             complement(5163..5978)
                     /codon_start=1
                     /product="kanamycin resistance protein"
                     /label=kanamycin resistance protein
                     /note="NptI"
                     /protein_id="AYC81466.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6104..6766)
                     /codon_start=1
                     /product="replication protein"
                     /label=replication protein
                     /protein_id="AYC81467.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      6767..7538
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"