LOCUS       Exported               12857 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pLX-CaMV, complete sequence.
ACCESSION   MH200606
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12857)
  AUTHORS   Pasin F, Tseng XA, Bedoya LC, Heydarnejad J, Deng TC, Garcia JA, 
            Chen YR.
  TITLE     Streamlined generation of plant virus infectious clones using the 
            pLX mini binary vectors
  JOURNAL   J. Virol. Methods (2018) In press
  PUBMED    30236898
REFERENCE   2  (bases 1 to 12857)
  AUTHORS   Pasin F.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2018) Agricultural Biotechnology Research Center, 
            Academia Sinica, 128 Academia Road, Section 2, Nangang District, 
            Taipei, Taipei 11529, Taiwan
REFERENCE   3  (bases 1 to 12857)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: UltraCycler v. v1.0
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12857
                     /organism="Binary vector pLX-CaMV"
                     /mol_type="other DNA"
                     /db_xref="taxon:2364170"
     regulatory      25..72
                     /regulatory_class="terminator"
                     /note="bacterial terminator"
     misc_feature    133..157
                     /label=LB
                     /note="LB"
     misc_feature    180..210
                     /label=LB
                     /note="LB"
     misc_feature    184..208
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      260..1203
                     /regulatory_class="promoter"
                     /note="CaMV 35S promoter"
     promoter        862..1206
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    1204..9902
                     /note="Cauliflower mosaic virus isolate Cabbage B-John 
                     Innes (1.08x); KJ716236"
     polyA_signal    1251..1427
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             2168..3151
                     /codon_start=1
                     /product="movement protein"
                     /label=movement protein
                     /protein_id="AYC81468.1"
                     /translation="MDLYPEENTQSEQSQNSENNMQIFKSENSDGFSSDLMISNDQLKN
                     ISKTQLTLEKEKIFKMPNVLSQVMKKAFSRKNEIHYCVSTKELSVDIHDATGKVYLPLI
                     TKEEINKRLSSLKPEVRKTMSMVHLGAVKILLKAQFRNGIDTPIKIALIDDRINSRKDC
                     LLGAAKGNLAYGKFMFTVYPKFGISLNTQRLNQTLSLIHDFENKNLMNKGDKVMTITYI
                     VGYALTNSHHSIDYQSNATIELEDVFQEIGNVQQSDFCTIQNDECNWAIDIAQNKALLG
                     AKAKTQIGNSLQIGNGASSSNTENELARVSQNIDLLKNKLKEICGE"
     CDS             3154..3633
                     /codon_start=1
                     /product="aphid transmission protein"
                     /label=aphid transmission protein
                     /protein_id="AYC81469.1"
                     /translation="MSITGQPHVYKKDTIIRLKPLSLNSNNRSYVFSSSKGNIQNIINH
                     LNNLNEIVGRSLLGIWKINSYFGLSKDPSESKSKNPSVFNTAKTIFKSGGVDYSSQLKE
                     IKSLLESQNTRIKSLEKAIQSLDDKIEPEPLTKEEVKELKESINSIKEGLKNIIG"
     CDS             3635..4024
                     /codon_start=1
                     /product="DNA-binding protein"
                     /label=DNA-binding protein
                     /protein_id="AYC81470.1"
                     /translation="MANLNQIQKEVSEILSDQKSMKADIKAILELLGSQNPIKESLEAV
                     AAKIVNDLSKLINDCPCNKEILEALGNQPKEQLIEQPKEKGKGLNLGKYSYPNYGVGNE
                     ELGSSGNPKALTWPFKAPAGWPNQF"
     CDS             4006..5469
                     /codon_start=1
                     /product="capsid protein"
                     /label=capsid protein
                     /protein_id="AYC81471.1"
                     /translation="MAESILDRTINRFWYNLGEDCLSESQFDLMIRLMEESLDGDQIID
                     LTSLPSDNLQVEQVMTTTEDSISEESEFLLAIGETSEDESDSGEEPEFEQVRMDRTGGT
                     EIPKEEDGEPSRYNERKRKTPEDRYFPTQPKTIPGQKQTSMGMLNIDCQTNRRTLIDDW
                     AAEIGLIVKTNREDYLDPETILLLMEHKTSGIAKELIRNTRWNRTTGDIIEQVIDAMYT
                     MFLGLNYSDNKVAEKIDEQEKAKIRMTKLQLCDICYLEEFTCDYEKNMYKTELADFPGY
                     INQYLSKIPIIGEKALTRFRHEANGTSTYSLGFAAKIVKEELSKICDLSKKQKKLKKFN
                     KKCCSIGEASVEYGCKKTSKKKYHKRYKKKYKVYKPYKKKKKFRSGKYFKPKEKKGSKQ
                     KYCPKGKKDCRCWICNIEGHYANECPNRQSSEKAHILQQAENLGLQPIEEPYEGVQEVF
                     ILEYKEEEEETSTEESDDGSSTSEDSDSD"
     CDS             5429..7471
                     /codon_start=1
                     /product="reverse transcriptase"
                     /label=reverse transcriptase
                     /protein_id="AYC81472.1"
                     /translation="MMDHLLLKTQTQTEQVMNVTNPNSIYIKGRLYFKGYKKIELHCFV
                     DTGASLCIASKFVIPEEHWVNAERPILVKIADGSSITISKVCKDIDLIIAGEIFKIPTV
                     YQQESGIDFIIGNNFCQLYEPFIQFTDRVIFTKNKSYPVHIAKLTRAVRVGTEGFLESM
                     KKRSKTQQPEPVNISTNKIENPLEEIAILSEGRRLSEEKLFITQQRMQKIEELLEKVCS
                     ENPLDPNKTKQWMKASIKLSDPSKAIKVKPMKYSPMDREEFDKQIKELLDLKVIKPSKS
                     PHMAPAFLVNNEAEKRRGKKRMVVNYKAMNKATIGDAYNLPNKDELLTLIRGKKIFSSF
                     DCKSGFWQVLLDQESRPLTAFTCPQGHYEWNVVPFGLKQAPSIFQRHMDEAFRVFRKFC
                     CVYVDDILVFSNNEEDHLLHVAMILQKCNQHGIILSKKKAQLFKKKINFLGLEIDEGTH
                     KPQGHILEHINKFPDTLEDKKQLQRFLGILTYASDYIPKLAQIRKPLQAKLKENVPWKW
                     TKEDTLYMQKVKKNLQGFPPLHHPLPEEKLIIETDASDDYWGGMLKAIKINEGTNTELI
                     CRYASGSFKAAEKNYHSNDKETLAVINTIKKFSIYLTPVHFLIRTDNTHFKSFVNLNYK
                     GDSKLGRNIRWQAWLSHYSFDVEHIKGTDNHFADFLSREFNKVNS"
     CDS             7575..9137
                     /codon_start=1
                     /product="inclusion body protein"
                     /label=inclusion body protein
                     /protein_id="AYC81473.1"
                     /translation="MENIEKLLMQEKILMLELDLVRAKISLARANGSSQQGDLPLHRET
                     PVKEEAVHSALATFTPTQVKAIPEQTAPGKESTNPLMANILPKDMNPVQTGIRLAVPED
                     FLRPHQGIPIPQKSELNSTVAPLRAESGIQHPHINYYVVYNGPHAGIYDDWGCTKAATN
                     GVPGVAHKKFATITEARAAADAYTTSQQTDRLNFIPKGEAQLKPKSFAKALTSPPKQKA
                     HWLTLGTKRPSSDPAPKEISFAPEITMDDFLYLYDLGRKFEGEGDDTMFTTDNEKVSLF
                     NFRKNADPQMVREAYAAGLIKTIYPSNNLQEIKYLPKKVKDAVKRFRTNCIKNTEKDIF
                     LKIRSTIPVWTIQGLLHKPRQVIEIGVSKKVVPTESKAMESKIQIEDLTELAVKTGEQF
                     IQSLLRLNDKKKIFVNMVEHDTLVYSKNVKDTVSEDQRAIETFQQRVISGNLLGFHCPA
                     ICHFIERTVEKEGGSYKCHHCDKGKAIIQDASADSGPKDGPPPTRSIVEKEDVPTTSSK
                     QVD"
     promoter        8891..9236
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    9281..9457
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     regulatory      9903..10158
                     /regulatory_class="terminator"
                     /note="nos terminator"
     terminator      9906..10158
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    10223..10246
                     /label=RB
                     /note="RB"
     regulatory      10311..10351
                     /regulatory_class="terminator"
                     /note="bacterial terminator"
     CDS             complement(10395..11210)
                     /codon_start=1
                     /product="kanamycin resistance protein"
                     /label=kanamycin resistance protein
                     /note="NptI"
                     /protein_id="AYC81474.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(11336..11998)
                     /codon_start=1
                     /product="replication protein"
                     /label=replication protein
                     /protein_id="AYC81475.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      11999..12770
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"