LOCUS       Exported                9943 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pLvCmvMYOCD2aHA, complete sequence.
ACCESSION   EU000249
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9943)
  AUTHORS   van Tuyn J, Sluiter JP, Swildens J, Goumans M-J., Doevendans PA, van
            der Laarse A, Schalij MJ, Atsma DE, de Vries AA.
  TITLE     Gene expression profiling of myocardin-transduced human cardiac 
            progenitor cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9943)
  AUTHORS   van Tuyn J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2007) MCB, LUMC, Einthovenweg 20, Leiden, ZH 
            2333ZH, the Netherlands
REFERENCE   3  (bases 1 to 9943)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9943
                     /organism="Shuttle vector pLvCmvMYOCD2aHA"
                     /mol_type="other DNA"
                     /db_xref="taxon:454257"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     repeat_region   212..410
     LTR             230..410
                     /label=5 LTR (truncated)
                     /note="truncated 5 long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    462..601
                     /label=packaging signal
                     /note="packaging signal"
     misc_feature    928..1785
                     /label=RRE
                     /note="RRE"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1799..1916
                     /label=CPPt
                     /note="CPPt"
     regulatory      1939..2514
                     /regulatory_class="promoter"
                     /note="CMV.IE"
     enhancer        1939..2242
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2243..2446
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     gene            2650..5693
                     /gene="MYOCD"
                     /label=MYOCD
     CDS             2650..2775
                     /codon_start=1
                     /gene="MYOCD"
                     /product="truncated cardiac myocardin"
                     /label=MYOCD
                     /note="N-terminal region"
                     /protein_id="ABS20111.1"
                     /translation="MTLLGSEHSLLIRSKFRSVLQLRLQQRRTQEQLANQGIIPH"
     CDS             2931..5693
                     /codon_start=1
                     /gene="MYOCD"
                     /product="smooth muscle myocardin"
                     /label=MYOCD
                     /note="smooth muscle enriched isoform of myocardin"
                     /protein_id="ABS20112.1"
                     /translation="MHILQASTAERSIPTAQMKLKRARLADDLNEKIALRPGPLELVEK
                     NILPVDSAVKEAIKGNQVSFSKSTDAFAFEEDSSSDGLSPDQTRSEDPQNSAGSPPDAK
                     ASDTPSTGSLGTNQDLASGSENDRNDSASQPSHQSDAGKQGLGPPSTPIAVHAAVKSKS
                     LGDSKNRHKKPKDPKPKVKKLKYHQYIPPDQKAEKSPPPMDSAYARLLQQQQLFLQLQI
                     LSQQQQQQQHRFSYLGMHQAQLKEPNEQMVRNPNSSSTPLSNTPLSPVKNSFSGQTGVS
                     SFKPGPLPPNLDDLKVSELRQQLRIRGLPVSGTKTALMDRLRPFQDCSGNPVPNFGDIT
                     TVTFPVTPNTLPNYQSSSSTSALSNGFYHFGSTSSSPPISPASSDLSVAGSLPDTFNDA
                     SPSFGLHPSPVHVCTEESLMSSLNGGSVPSELDGLDSEKDKMLVEKQKVINELTWKLQQ
                     EQRQVEELRMQLQKQKRNNCSEKKPLPFLAASIKQEEAVSSCPFASQVPVKRQSSSSEC
                     HPPACEAAQLQPLGNAHCVESSDQTNVLSSTFLSPQCSPQHSPLGAVKSPQHISLPPSP
                     NNPHFLPSSSGAQGEGHRVSSPISSQVCTAQNSGAHDGHPPSFSPHSSSLHPPFSGAQA
                     DSSHGAGGNPCPKSPCVQQKMAGLHSSDKVGPKFSIPSPTFSKSSSAISEVTQPPSYED
                     AVKQQMTRSQQMDELLDVLIESGEMPADAREDHSCLQKVPKIPRSSRSPTAVLTKPSAS
                     FEQASSGSQIPFDPYATDSDEHLEVLLNSQSPLGKMSDVTLLKIGSEEPHFDGIMDGFS
                     GKAAEDLFNAHEILPGPLSPMQTQFSPSSVDSNGLQLSFTESPWETMEWLDLTPPNSTP
                     GFSALTTSSPSIFNIDFLDVTDLNLNSSMDLHLQQWTGGYPYDVPDYAL"
     misc_feature    5661..5690
                     /gene="MYOCD"
                     /label=Region: HA-tag
                     /note="Region: HA-tag"
     CDS             5661..5687
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    5730..6321
                     /label=WPRE
                     /note="WPRE"
     misc_feature    5730..6318
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6201..6212)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6457..6690
                     /label=3 LTR (Delta-U3)
                     /note="self-inactivating 3 long terminal repeat (LTR) from
                     HIV-1"
     repeat_region   6491..6692
     polyA_signal    6762..6883
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6923..7058
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7079..7097)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7107..7123)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      7268..7723
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7752..7856
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7857..8717
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="ampicillin resistance"
                     /protein_id="ABS20113.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8888..9476
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    9764..9785
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9801..9831
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9839..9855
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9863..9879
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9900..9918
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"