LOCUS       Exported                6271 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLV.MCS.WHVPRE, complete sequence.
ACCESSION   JN622008
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Ramkisoensing AA, Swildens J, Atsma DE, Pijnappels DA, Schalij MJ, 
            de Vries AAF.
  TITLE     Gap junctional coupling with cultured neonatal rat cardiomyocytes 
            induces the cardiomyogenic differentiation of fetal human 
            mesenchymal stem cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6271)
  AUTHORS   Ramkisoensing AA, Swildens J, Atsma DE, Pijnappels DA, Schalij MJ, 
            de Vries AAF.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2011) Cardiology, Leiden University Medical 
            Center, Albinusdreef 2, Leiden, Zuid-Holland 2333 ZA, The 
            Netherlands
REFERENCE   3  (bases 1 to 6271)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Cloning vector pLV.MCS.WHVPRE"
                     /mol_type="other DNA"
                     /db_xref="taxon:1135949"
     repeat_region   1..410
                     /note="hybrid 5 LTR derived from Rous sarcoma virus and 
                     HIV-1 LTR sequences"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5 LTR (truncated)
                     /note="truncated 5 long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     regulatory      473..588
                     /regulatory_class="other"
                     /note="HIV-1 packaging signal"
     misc_feature    928..1785
                     /label=RRE
                     /note="RRE"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1799..1916
                     /label=cPPT
                     /note="cPPT"
     misc_feature    1917..2053
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      2058..2649
                     /regulatory_class="other"
                     /note="WHVPRE"
     misc_feature    2058..2646
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(2529..2540)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     repeat_region   2650..3018
                     /label=self-inactivating 3 LTR
                     /note="self-inactivating 3 LTR"
     LTR             2785..3018
                     /label=3 LTR (Delta-U3)
                     /note="self-inactivating 3 long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3090..3211
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      3251..3386
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(3407..3425)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3435..3451)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3596..4051
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4080..4184
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4185..5045
                     /codon_start=1
                     /gene="AMPR"
                     /product="beta-lactamase"
                     /label=AMPR
                     /note="ampicillin resistance protein"
                     /protein_id="AEY75226.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            4185..5045
                     /gene="AMPR"
                     /label=AMPR
     rep_origin      5216..5804
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6092..6113
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6129..6159
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6167..6183
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6191..6207
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6228..6246
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"