LOCUS       Exported                4719 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Low threshold vector pLTSUB-302, complete sequence.
ACCESSION   AY962288
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Basu S, Gerchman Y, Collins CH, Arnold FH, Weiss R.
  TITLE     A synthetic multicellular system for programmed pattern formation
  JOURNAL   Nature 434 (7037), 1130-1134 (2005)
  PUBMED    15858574
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Basu S, Weiss R.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAR-2005) Electrical Engineering, Princeton 
            University, J-319, E-quad, Olden Street, Princeton, NJ 08544, USA
REFERENCE   3  (bases 1 to 4719)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Low threshold vector pLTSUB-302"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:321071"
     regulatory      1..74
                     /regulatory_class="promoter"
                     /note="Luxp(r); Lux promoter"
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             115..828
                     /codon_start=1
                     /gene="cI"
                     /product="lambda repressor protein"
                     /label=cI
                     /protein_id="AAX63486.1"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     gene            115..828
                     /gene="cI"
                     /label=cI
     RBS             886..897
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             904..1623
                     /codon_start=1
                     /gene="ecfp"
                     /product="cyan flourescent protein"
                     /label=ecfp
                     /protein_id="AAX63487.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     gene            904..1623
                     /gene="ecfp"
                     /label=ecfp
     terminator      1647..1733
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             1848..1859
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1866..2948
                     /codon_start=1
                     /gene="lacI"
                     /product="LacI repressor protein"
                     /label=lacI
                     /protein_id="AAX63488.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     gene            1866..2948
                     /gene="lacI"
                     /label=lacI
     CDS             3103..3897
                     /codon_start=1
                     /gene="kan(r)"
                     /product="kanamycin resistance protein"
                     /label=kan(r)
                     /note="antibiotic resistance"
                     /protein_id="AAX63489.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            3103..3897
                     /gene="kan(r)"
                     /label=kan(r)
     terminator      3928..4022
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      4136..4681
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."