LOCUS       Exported                5393 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLT-26, complete sequence.
ACCESSION   KX272604
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Tian L, Lo J, Shao X, Zheng T, Olson DG, Lynd LR.
  TITLE     Identification of a ferredoxin:NAD+ oxidoreductase enzyme in 
            Thermoanaerobacterium saccharolyticum and its role in ethanol 
            formation
  JOURNAL   Appl. Environ. Microbiol. (2016) In press
  PUBMED    27694237
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Tian L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2016) Thayer School of Engineering, Dartmouth 
            College, 14 Engineering Dr, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 5393)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Cloning vector pLT-26"
                     /mol_type="other DNA"
                     /db_xref="taxon:1906602"
     rep_origin      66..654
     CDS             complement(825..1685)
                     /codon_start=1
                     /product="ampicillin resistance protein"
                     /label=ampicillin resistance protein
                     /protein_id="AOU74532.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1686..1790)
                     /gene="bla"
                     /label=AmpR promoter
     misc_recomb     1902..2371
     misc_recomb     2371..2372
     CDS             3377..4138
                     /codon_start=1
                     /product="HTK"
                     /label=HTK
                     /note="KanR"
                     /protein_id="AOU74533.1"
                     /translation="MKGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGRQTD
                     GPYSDIEMMCVLSTEGVEFSYEWTTGEWKAEVNFYSEEILLDYASRVEPDWPLTHGRFF
                     SILPIYDPGGYFEKVYQTAKSVEAQKFHDAICALIVEELFEYAGKWRNIRVQGPTTFLP
                     SLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQPDLPPGYVQLCQLVMSGQLSDPEK
                     LLESLENFWNGVQEWAERHGYIVDVSKRIPF"
     CDS             4162..4743
                     /codon_start=1
                     /product="TDK"
                     /label=TDK
                     /note="TheetDRAFT_0051"
                     /protein_id="AOU74531.1"
                     /translation="MIYGSLDHGFIEAIVGPMFSGKSEELIRRIKRAQIAKQKVQVFKP
                     AIDDRYSIDKVVSHNGTNINAISVVKASEIIELLEEDTEVIAIDEIQFFDHSIVDVVRE
                     IADLGKRVICAGLDMDFRGEPFGPTPDIMAIAESVDKLTAICVKCGNPATRTQRLINGK
                     PAKYDDPIILVGAHETYEARCRKCHEVPRT"
     misc_recomb     4822..5283