LOCUS       Exported                5034 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pLSU-11, complete sequence.
ACCESSION   HQ608522
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Lee S.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2010) Plant Pathology and Crop Physiology, 
            Louisiana State University, 302 Life Science Bldg, Baton Rouge, LA 
            70820, USA
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Binary vector pLSU-11"
                     /mol_type="other DNA"
                     /db_xref="taxon:1089839"
     CDS             95..1285
                     /codon_start=1
                     /gene="tetA"
                     /product="TetA"
                     /label=tetA
                     /note="tetracycline resistance protein"
                     /protein_id="AEP34034.1"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     gene            95..1285
                     /gene="tetA"
                     /label=tetA
     rep_origin      1484..2072
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      1515..2071
                     /label=ColE1 replication origin
                     /note="ColE1 replication origin"
     misc_binding    2145..2167
                     /label=dnaA binding site
                     /bound_moiety="dnaA"
     CDS             2314..2943
                     /codon_start=1
                     /gene="staA"
                     /product="StaA"
                     /label=staA
                     /protein_id="AEP34033.1"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     gene            2314..2943
                     /gene="staA"
                     /label=staA
     CDS             3372..4445
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /note="from pVS1 plasmid"
                     /protein_id="AEP34035.1"
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELVDWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     gene            3372..4445
                     /gene="repA"
                     /label=repA
     rep_origin      4511..4705
                     /label=VS1 replication origin
                     /note="VS1 replication origin"
     misc_feature    4872..4895
                     /label=T-DNA left border repeat
                     /note="T-DNA left border repeat"
     misc_feature    4968..5029
                     /label=T-DNA right border repeat
                     /note="T-DNA right border repeat"