LOCUS       Exported                4566 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pLSU-1, complete sequence.
ACCESSION   HQ608521
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Lee S, Su G, Lasserre E, Aghazadeh MA, Murai N.
  TITLE     Small high-yielding binary Ti vectors pLSU with co-directional 
            replicons for Agrobacterium tumefaciens-mediated transformation of 
            higher plants
  JOURNAL   Plant Sci. 187, 49-58 (2012)
  PUBMED    22404832
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Lee S.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2010) Plant Pathology and Crop Physiology, 
            Louisiana State University, 302 Life Science Bldg, Baton Rouge, LA 
            70820, USA
REFERENCE   3  (bases 1 to 4566)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Binary vector pLSU-1"
                     /mol_type="other DNA"
                     /db_xref="taxon:1089838"
     CDS             111..926
                     /codon_start=1
                     /gene="nptI"
                     /product="neomycin phosphotransferase"
                     /label=nptI
                     /protein_id="AEP34030.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            111..926
                     /gene="nptI"
                     /label=nptI
     rep_origin      1012..1600
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      1044..1600
                     /label=ColE1 replication origin
                     /note="ColE1 replication origin"
     misc_binding    1673..1695
                     /label=dnaA binding site
                     /bound_moiety="dnaA"
     CDS             1846..2475
                     /codon_start=1
                     /gene="staA"
                     /product="StaA"
                     /label=staA
                     /protein_id="AEP34031.1"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     gene            1846..2475
                     /gene="staA"
                     /label=staA
     gene            2802..3977
                     /gene="repA"
                     /label=repA
     CDS             2904..3977
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /note="pVS1 plasmid"
                     /protein_id="AEP34032.1"
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELVDWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      4043..4237
                     /label=VS1 replication origin
                     /note="VS1 replication origin"
     misc_feature    4404..4427
                     /label=T-DNA left border repeat
                     /note="T-DNA left border repeat"
     misc_feature    4500..4561
                     /label=T-DNA right border repeat
                     /note="T-DNA right border repeat"