LOCUS       Exported                3451 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLS975, complete sequence.
ACCESSION   GU074395
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma C, Shang GD.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2009) College of Life Sciences, Nanjing Normal 
            University, No. 1 Wenyuan Rd., XiXia District, Nanjing, Jiangsu 
            210046, P.R. China
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Cloning vector pLS975"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:686799"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             779..1249
                     /codon_start=1
                     /gene="rpsL"
                     /product="synthetic 30S ribosomal subunit protein S12"
                     /label=rpsL
                     /protein_id="ACY66809.1"
                     /translation="MELHRGGGRSRTSGSEFDIKLIDTVDLEGGPGTATVNQLVRKPRA
                     RKVAKSNVPALEACPQKRGVCTRVYTTTPKKPNSALRKVCRVRLTNGFEVTSYIGGEGH
                     NLQEHSVILIRGGRVKDLPGVRYHTVRGALDCSGVKDRKQARSKYGVKRPKA"
     gene            779..1249
                     /gene="rpsL"
                     /label=rpsL
     misc_feature    782..877
                     /gene="rpsL"
                     /label=multiple cloning site
                     /note="multiple cloning site"
     primer_bind     806..822
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(844..860)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1263..1281)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1302..1318)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1326..1342
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1350..1380)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1395..1416
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1704..2292)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2463..3323)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="ACY66810.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2463..3323)
                     /gene="bla"
                     /label=bla
     promoter        complement(3324..3428)
                     /gene="bla"
                     /label=AmpR promoter