LOCUS       Exported                6270 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLR3, complete sequence.
ACCESSION   HQ007051
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Koksharov MI, Ugarova NN.
  TITLE     Random mutagenesis of Luciola mingrelica firefly luciferase. Mutant 
            enzymes with bioluminescence spectra showing low pH sensitivity
  JOURNAL   Biochemistry Mosc. 73 (8), 862-869 (2008)
  PUBMED    18774932
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   Koksharov MI, Ugarova NN.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2010) Department of Chemistry, Lomonosov Moscow 
            State University, Leninskie Gory, Bld. 1/11, GSP-3, Moscow 119991, 
            Russia
REFERENCE   3  (bases 1 to 6270)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Cloning vector pLR3"
                     /mol_type="other DNA"
                     /db_xref="taxon:910448"
     CDS             complement(5..814)
                     /codon_start=1
                     /product="LuxR"
                     /label=LuxR
                     /note="bacterial activator of transcription"
                     /protein_id="ADP02958.1"
                     /translation="MIYNTQNLRQTIGKDKEMGMKDINADDTYRIINKIKACRSNNDIN
                     QCLSDMTKMVHCEYYLLAIIYPHSMVKSDISILDNYPKKWRQYYDDANLIKYDPIVDYS
                     NSNHSPINWNIFENNAVNKKSPNVIKEAKSSGLITGFSFPIHTANNGFGMLSFAHSEKD
                     NYIDSLFLHACMNIPLIVPSLVDNYRKINIANNKSNNDLTKREKECLAWACEGKSSWDI
                     SKILGCSKRTVTFHLTNAQMKLNTTNRCQSISKAILTGAIDCPYFKS"
     CDS             937..1557
                     /codon_start=1
                     /product="LuxI"
                     /label=LuxI
                     /note="bacterial autoinductor"
                     /protein_id="ADP02959.1"
                     /translation="MVCYSRLNAREVGMTIMIKKSDFLAIPSEEYKGILSLRYQVFKQR
                     LEWDLVVENNLESDEYDNSNAEYIYACDDTENVSGCWRLLPTTGDYMLKSVFPELLGQQ
                     SAPKDPNIVELSRFAVGKNSSKINNSASEITMKLFEAIYKHAVSQGITEYVTVTSTAIE
                     RFLKRIKVPCHRIGDKEIHVLGDTKSVVLSMPINEQFKKAVLN"
     CDS             1615..3261
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=firefly luciferase
                     /note="Luc; derived from Luciola mingrelica firefly 
                     luciferase; contains 3 additional restriction sites (BstBI,
                     SalI, XhoI) in comparison to the wild-type sequence in INSD
                     accession S61961"
                     /protein_id="ADP02960.1"
                     /translation="MEMEKEENVVYGPLPFYPIEEGSAGIQLHKYMHQYAKLGAIAFSN
                     ALTGVDISYQEYFDITCRLAEAMKNFGMKPEEHIALCSENCEEFFIPVLAGLYIGVAVA
                     PTNEIYTLRELNHSLGIAQPTIVFSSRKGLPKVLEVQKTVTCIKKIVILDSKVNFGGHD
                     CMETFIKKHVELGFQPSSFVPIDVKNRKQHVALLMNSSGSTGLPKGVRITHEGAVTRFS
                     HAKDPIYGNQVSPGTAILTVVPFHHGFGMFTTLGYFACGYRVVMLTKFDEELFLRTLQD
                     YKCTSVILVPTLFAILNKSELIDKFDLSNLTEIASGGAPLAKEVGEAVARRFNLPGVRQ
                     GYGLTETTSAFIITPEGDDKPGASGKVVPLFKVKVIDLDTKKTLGVNRRGEICVKGPSL
                     MLGYSNNPEATRETIDEEGWLHTGDIGYYDEDEHFFIVDRLKSLIKYKGYQVPPAELES
                     VLLQHPNIFDAGVAGVPDPDAGELPGAVVVMEKGKTMTEKEIVDYVNSQVVNHKRLRGG
                     VRFVDEVPKGLTGKIDAKVIREILKKPQAKM"
     promoter        3436..3540
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      3471..3499
                     /regulatory_class="promoter"
                     /note="bla promoter"
     CDS             3541..4401
                     /codon_start=1
                     /product="Bla"
                     /label=Bla
                     /note="beta-lactamase; ampicillin resistance protein"
                     /protein_id="ADP02961.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4556..5175
                     /label=pBR322 origin
                     /note="pBR322 origin"
     rep_origin      4572..5160
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5346..5486
                     /label=bom
                     /note="basis of mobility region from pBR322"
     misc_feature    complement(5588..5779)
                     /label=Region: ROP
                     /note="Region: ROP"
     CDS             complement(5588..5779)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"