LOCUS       Exported                8416 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLN-ENR-GFP, complete sequence.
ACCESSION   DQ813653
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   Nkrumah LJ, Muhle RA, Moura PA, Ghosh P, Hatfull GF, Jacobs WR Jr., 
            Fidock DA.
  TITLE     Efficient site-specific integration in Plasmodium falciparum 
            chromosomes mediated by mycobacteriophage Bxb1 integrase
  JOURNAL   Nat. Methods 3 (8), 615-621 (2006)
  PUBMED    16862136
REFERENCE   2  (bases 1 to 8416)
  AUTHORS   Nkrumah LJ, Fidock DA.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2006) Microbiology and Immunology, Albert Einstein
            College of Medicine, 1300 Morris Park, Bronx, NY 10461, USA
REFERENCE   3  (bases 1 to 8416)
  AUTHORS   Nkrumah LJ, Fidock DA.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2007) Microbiology and Immunology, Albert Einstein
            College of Medicine, 1300 Morris Park, Bronx, NY 10461, USA
REFERENCE   4  (bases 1 to 8416)
  AUTHORS   Nkrumah LJ, Fidock DA.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2009) Microbiology and Immunology, Albert Einstein
            College of Medicine, 1300 Morris Park, Bronx, NY 10461, USA
REFERENCE   5  (bases 1 to 8416)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Jul 10, 2009 this sequence version replaced DQ813653.2.
FEATURES             Location/Qualifiers
     source          1..8416
                     /organism="Cloning vector pLN-ENR-GFP"
                     /mol_type="other DNA"
                     /db_xref="taxon:393301"
     regulatory      complement(40..876)
                     /regulatory_class="terminator"
                     /note="derived from hsp86 3 UTR"
     CDS             complement(882..2897)
                     /codon_start=1
                     /gene="enr-gfp"
                     /product="ENR-GFP"
                     /label=enr-gfp
                     /note="enoyl ACP reductase-GFP Mut2 fusion; PfENR; derived 
                     from Plasmodium falciparum"
                     /protein_id="ABG81366.2"
                     /translation="MDKISQRLLFLFLHFYTIVCFIQNNTQKTFHNVLQNEQIRGKEKA
                     FYRKEKRENIFIGNKMKHLNNMNNTHNNNHYMEKEEQDASNINKIKEENKNEDICFIAG
                     IGDTNGYGWGIAKELSKRNVKIIFGIWPPVYNIFMKNYKNGKFDNDMIIDKDKKMNILD
                     MLPFDASFDTANDIDEETKNNKRYNMLQNYTTEDVANLIHQKYGKINMLVHSLANAKEV
                     QKDLLNTSRKGYLDALSKSSYSLISLCKYFVNIMKPQSSIISLTYHASQKVVPGYGGGM
                     SSAKAALESDTRVLAYHLGRNYNIRINTISAGPLKSRAATAINKLNNTYENNTNQNKNR
                     NSHDVHNIMNNSGEKEEKKNSASQNYTFIDYAIEYSEKYAPLRQKLLSTDIGSVASFLL
                     SRESRAITGQTIYVDNGLNIMFLPDDIYRNENERTSKGEELFTGVVPILVELDGDVNGH
                     KFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFF
                     KSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEY
                     NYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLS
                     TQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK"
     gene            complement(882..2897)
                     /gene="enr-gfp"
                     /label=enr-gfp
     misc_feature    complement(882..1595)
                     /gene="enr-gfp"
                     /label=GFP
                     /note="GFP"
     CDS             complement(882..1595)
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    complement(1602..2897)
                     /gene="enr-gfp"
                     /label=ENR
                     /note="ENR"
     misc_feature    complement(2905..3916)
                     /label=contains promoter and 5 UTR from calmodulin gene
                     /note="contains promoter and 5 UTR from calmodulin gene"
     misc_feature    3999..4593
                     /label=contains promoter and 5 UTR from PcDT gene
                     /note="contains promoter and 5 UTR from PcDT gene"
     CDS             4605..5006
                     /codon_start=1
                     /gene="bsd"
                     /product="blasticidin S deaminase"
                     /label=bsd
                     /note="BSD; selectable marker"
                     /protein_id="ABG81367.1"
                     /translation="MHAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFT
                     GVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPG
                     IKAIVKDSDGQPTAVGIRELLPSGYVWEG"
     gene            4605..5006
                     /gene="bsd"
                     /label=bsd
     regulatory      5012..5608
                     /regulatory_class="terminator"
                     /note="derived from hrp2 3 UTR"
     misc_feature    complement(5622..5728)
                     /label=tandem attP elements
                     /note="tandem attP elements"
     misc_recomb     complement(5622..5672)
                     /label=attP
                     /note="attP"
     misc_recomb     complement(5679..5728)
                     /label=attP
                     /note="attP"
     promoter        complement(5734..5751)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5758..5774)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6248..6352
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6353..7213
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7384..7972
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    8260..8281
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8296..8326
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8334..8350
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8358..8374
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8392..8410
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"