LOCUS       Exported                6806 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLMF01, complete sequence.
ACCESSION   KU963002
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Liu M, Wang M.
  TITLE     Development of a plasmid-based methods for expression and antisense 
            gene knockdown in Riemerella anatipestifer and application
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6806)
  AUTHORS   Liu M, Wang M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2016) Institute of Preventive Veterinary Medicine,
            Sichuan Agricultural University, No. 211, Huimin Road, Chengdu, 
            Sichuan 611130, China
REFERENCE   3  (bases 1 to 6806)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6806
                     /organism="Cloning vector pLMF01"
                     /mol_type="other DNA"
                     /label=shuttle vector
                     /note="shuttle vector"
                     /db_xref="taxon:1882243"
     protein_bind    290..311
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        326..356
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    364..380
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     388..404
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2763..2780
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(3449..4414)
                     /codon_start=1
                     /product="beta-lactamase CfxA"
                     /function="cefoxitin resistance protein"
                     /label=beta-lactamase CfxA
                     /protein_id="ANW35655.1"
                     /translation="MEKNRKKQIVVLSIALVCIFILVFSLFHKSATKDSANPPLTNVLT
                     DSISQIVSACPGEIGVAVIVNNRDTVKVNNKSVYPMMSVFKVHQALALCNDFDNKGISL
                     DTLVNINRDKLDPKTWSPMLKDYSGPVISLTVRDLLRYTLTQSDNNASNLMFKDMVNVA
                     QTDSFIATLIPRSSFQIAYTEEEMSADHNKAYSNYTSPLGAAMLMNRLFTEGLIDDEKQ
                     SFIKNTLKECKTGVDRIAAPLLDKEGVVIAHKTGSGYVNENGVLAAHNDVAYICLPNNI
                     SYTLAVFVKDFKGNKSQASQYVAHISAVVYSLLMQTSVKS"
     primer_bind     complement(4594..4610)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5084..5188
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5189..6049
                     /codon_start=1
                     /product="beta-lactamase"
                     /function="ampicillin resistance protein"
                     /label=beta-lactamase
                     /protein_id="ANW35654.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6220..2
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"